The nuclear 16-kD protein methylation increases in the early period of liver regeneration in a hepatectomized rat

Kyounghwa Lee, Kyung Mi Lee, Tae Jin Kim, Meyoung-Kon Kim, Jong Seol Han, Yoon Sik Hong, Gil-Hong Park, Kyo Won Lee

Research output: Contribution to journalArticlepeer-review


Methylation events play a critical role in various cellular processes including regulation of gene transcription and proliferation. We observed that methyltransferase activity underwent time-dependent changes in the cytosol of the rat hepatocytes upon partial hepatectomy. However, any change in the methylation of nuclear proteins is not clear during hepatocyte proliferation. The nuclear fraction possesses basal level of methyltransferase to catalyze methylation of several proteins ranging from 7 to 70 kD prior to any hepatecmony. The specific p16 (16 kD) band was transiently and heavily methylated post 1 day hepatectomy, and then became non-detectable, but not in the control liver. Methylation of p16 band was completely inhibited by exogenously added histones, particularly 2AS, 1, 2A and 2B subtypes. The methylated p16 protein remains stable in either acid or alkali- induced demethylation conditions, indicating that methylation is not likely to occur on isoaspartyl or C-terminal cysteinyl residues. Exogenous addition of non-hydrolyzable GTP caused a dose-dependent suppression of a p16 methylation suggesting that G-proteins might play a role as an endogenous methylation inhibitor in vivo. Taken together, we have identified the proliferation event associated-methylation of the nuclear p16 protein in the hepatocytes undergoing liver regeneration.

Original languageEnglish
Pages (from-to)563-571
Number of pages9
JournalExperimental and Molecular Medicine
Issue number6
Publication statusPublished - 2004 Dec 31


  • Histone
  • Nuclear methyl acceptors
  • Protein methylation
  • Regenerating rat liver

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry


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