The ribonuclease l-dependent antiviral roles of human 2′,5′- oligoadenylate synthetase family members against hepatitis C virus

Young Chan Kwon; Ju Il Kang; Soon B. Hwang; Byung Yoon Ahn

doi:10.1016/j.febslet.2012.11.010

The ribonuclease l-dependent antiviral roles of human 2′,5′- oligoadenylate synthetase family members against hepatitis C virus

Young Chan Kwon, Ju Il Kang, Soon B. Hwang, Byung Yoon Ahn

Research output: Contribution to journal › Article › peer-review

50 Citations (Scopus)

Abstract

The latent ribonuclease RNase L and the interferon-inducible 2′,5′-oligoadenylate synthetase (OAS) have been implicated in the antiviral response against hepatitis C virus (HCV). However, the specific roles of these enzymes against HCV have not been fully elucidated. In this study, a scarce endogenous expression and RNA degrading activity of RNase L in human hepatoma Huh7 cells enabled us to demonstrate the antiviral activity of RNase L against HCV replication through the transient expression of the enzyme. The antiviral potential of specific members of the OAS family was further examined through overexpression and RNA interference approaches. Our data suggested that among the members of the OAS family, OAS1 p46 and OAS3 p100 mediate the RNase l-dependent antiviral activity against HCV.

Original language	English
Pages (from-to)	156-164
Number of pages	9
Journal	FEBS Letters
Volume	587
Issue number	2
DOIs	https://doi.org/10.1016/j.febslet.2012.11.010
Publication status	Published - 2013 Jan 16

Bibliographical note

Funding Information:
This work was supported by the Basic Science Research Program of the National Research Foundation (#2012-008126) of the Republic of Korea. Y.C.K and J.I.K were supported by the BK21 Program from the Ministry of Education, Science & Technology of Korea.

Keywords

2′,5′-Oligoadenylate synthetase
Antiviral protein
Hepatitis C virus
RNase L

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.febslet.2012.11.010

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title = "The ribonuclease l-dependent antiviral roles of human 2′,5′- oligoadenylate synthetase family members against hepatitis C virus",

abstract = "The latent ribonuclease RNase L and the interferon-inducible 2′,5′-oligoadenylate synthetase (OAS) have been implicated in the antiviral response against hepatitis C virus (HCV). However, the specific roles of these enzymes against HCV have not been fully elucidated. In this study, a scarce endogenous expression and RNA degrading activity of RNase L in human hepatoma Huh7 cells enabled us to demonstrate the antiviral activity of RNase L against HCV replication through the transient expression of the enzyme. The antiviral potential of specific members of the OAS family was further examined through overexpression and RNA interference approaches. Our data suggested that among the members of the OAS family, OAS1 p46 and OAS3 p100 mediate the RNase l-dependent antiviral activity against HCV.",

keywords = "2′,5′-Oligoadenylate synthetase, Antiviral protein, Hepatitis C virus, RNase L",

author = "Kwon, {Young Chan} and Kang, {Ju Il} and Hwang, {Soon B.} and Ahn, {Byung Yoon}",

note = "Funding Information: This work was supported by the Basic Science Research Program of the National Research Foundation (#2012-008126) of the Republic of Korea. Y.C.K and J.I.K were supported by the BK21 Program from the Ministry of Education, Science & Technology of Korea. ",

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doi = "10.1016/j.febslet.2012.11.010",

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AU - Kang, Ju Il

AU - Hwang, Soon B.

AU - Ahn, Byung Yoon

N1 - Funding Information: This work was supported by the Basic Science Research Program of the National Research Foundation (#2012-008126) of the Republic of Korea. Y.C.K and J.I.K were supported by the BK21 Program from the Ministry of Education, Science & Technology of Korea.

PY - 2013/1/16

Y1 - 2013/1/16

N2 - The latent ribonuclease RNase L and the interferon-inducible 2′,5′-oligoadenylate synthetase (OAS) have been implicated in the antiviral response against hepatitis C virus (HCV). However, the specific roles of these enzymes against HCV have not been fully elucidated. In this study, a scarce endogenous expression and RNA degrading activity of RNase L in human hepatoma Huh7 cells enabled us to demonstrate the antiviral activity of RNase L against HCV replication through the transient expression of the enzyme. The antiviral potential of specific members of the OAS family was further examined through overexpression and RNA interference approaches. Our data suggested that among the members of the OAS family, OAS1 p46 and OAS3 p100 mediate the RNase l-dependent antiviral activity against HCV.

AB - The latent ribonuclease RNase L and the interferon-inducible 2′,5′-oligoadenylate synthetase (OAS) have been implicated in the antiviral response against hepatitis C virus (HCV). However, the specific roles of these enzymes against HCV have not been fully elucidated. In this study, a scarce endogenous expression and RNA degrading activity of RNase L in human hepatoma Huh7 cells enabled us to demonstrate the antiviral activity of RNase L against HCV replication through the transient expression of the enzyme. The antiviral potential of specific members of the OAS family was further examined through overexpression and RNA interference approaches. Our data suggested that among the members of the OAS family, OAS1 p46 and OAS3 p100 mediate the RNase l-dependent antiviral activity against HCV.

KW - 2′,5′-Oligoadenylate synthetase

KW - Antiviral protein

KW - Hepatitis C virus

KW - RNase L

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DO - 10.1016/j.febslet.2012.11.010

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AN - SCOPUS:84872109295

SN - 0014-5793

VL - 587

SP - 156

EP - 164

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

The ribonuclease l-dependent antiviral roles of human 2′,5′- oligoadenylate synthetase family members against hepatitis C virus

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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