The role of 14-3-3β in transcriptional activation of estrogen receptor α and its involvement in proliferation of breast cancer cells

Yoonseo Kim; Hyungjin Kim; Sung Wuk Jang; Jesang Ko

doi:10.1016/j.bbrc.2011.09.056

The role of 14-3-3β in transcriptional activation of estrogen receptor α and its involvement in proliferation of breast cancer cells

Yoonseo Kim
, Hyungjin Kim
, Sung Wuk Jang
, Jesang Ko^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

The estrogen receptor (ER) functions as a transcription factor that mediates the effects of estrogen. ERα, which plays a crucial role in the development and progression of breast cancer, is activated by estrogen binding, leading to receptor phosphorylation, dimerization, and recruitment of co-activators and chaperons to the estrogen-bound receptor complex. The 14-3-3 proteins bind to target proteins via phosphorylation and influence many cellular events by altering their subcellular localization or acting as a chaperone. However, regulation of ERα expression and transactivation by the 14-3-3 proteins has not been reported. We demonstrate that 14-3-3β functions as a positive regulator of ERα through a direct protein-protein interaction in an estrogen-dependent manner. Ectopic expression of 14-3-3β stimulated ERα-mediated transcriptional activity in MCF-7 breast cancer cells. Enhanced ERα transcriptional activity due to 14-3-3β increased the expressions of the endogenous ERα target genes, leading to proliferation of breast cancer cells. We suggest that 14-3-3β has oncogenic potential in breast cancer via binding to ERα and activation of the transcriptional activity of ERα.

Original language	English
Pages (from-to)	199-204
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	414
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2011.09.056
Publication status	Published - 2011 Oct 14

Bibliographical note

Funding Information:
This work was supported by the Basic Science Research Program ( 2011-0017957 ), the Basic Research Laboratory Program ( 2011-0001573 ), and the Bio & Medical Technology Development Program ( 2011-0027756 ) from the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology, Republic of Korea.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

14-3-3
Breast cancer
Estrogen receptor

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2011.09.056

Cite this

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title = "The role of 14-3-3β in transcriptional activation of estrogen receptor α and its involvement in proliferation of breast cancer cells",

abstract = "The estrogen receptor (ER) functions as a transcription factor that mediates the effects of estrogen. ERα, which plays a crucial role in the development and progression of breast cancer, is activated by estrogen binding, leading to receptor phosphorylation, dimerization, and recruitment of co-activators and chaperons to the estrogen-bound receptor complex. The 14-3-3 proteins bind to target proteins via phosphorylation and influence many cellular events by altering their subcellular localization or acting as a chaperone. However, regulation of ERα expression and transactivation by the 14-3-3 proteins has not been reported. We demonstrate that 14-3-3β functions as a positive regulator of ERα through a direct protein-protein interaction in an estrogen-dependent manner. Ectopic expression of 14-3-3β stimulated ERα-mediated transcriptional activity in MCF-7 breast cancer cells. Enhanced ERα transcriptional activity due to 14-3-3β increased the expressions of the endogenous ERα target genes, leading to proliferation of breast cancer cells. We suggest that 14-3-3β has oncogenic potential in breast cancer via binding to ERα and activation of the transcriptional activity of ERα.",

keywords = "14-3-3, Breast cancer, Estrogen receptor",

author = "Yoonseo Kim and Hyungjin Kim and Jang, \{Sung Wuk\} and Jesang Ko",

note = "Funding Information: This work was supported by the Basic Science Research Program ( 2011-0017957 ), the Basic Research Laboratory Program ( 2011-0001573 ), and the Bio \& Medical Technology Development Program ( 2011-0027756 ) from the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology, Republic of Korea.",

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AU - Ko, Jesang

N1 - Funding Information: This work was supported by the Basic Science Research Program ( 2011-0017957 ), the Basic Research Laboratory Program ( 2011-0001573 ), and the Bio & Medical Technology Development Program ( 2011-0027756 ) from the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology, Republic of Korea.

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N2 - The estrogen receptor (ER) functions as a transcription factor that mediates the effects of estrogen. ERα, which plays a crucial role in the development and progression of breast cancer, is activated by estrogen binding, leading to receptor phosphorylation, dimerization, and recruitment of co-activators and chaperons to the estrogen-bound receptor complex. The 14-3-3 proteins bind to target proteins via phosphorylation and influence many cellular events by altering their subcellular localization or acting as a chaperone. However, regulation of ERα expression and transactivation by the 14-3-3 proteins has not been reported. We demonstrate that 14-3-3β functions as a positive regulator of ERα through a direct protein-protein interaction in an estrogen-dependent manner. Ectopic expression of 14-3-3β stimulated ERα-mediated transcriptional activity in MCF-7 breast cancer cells. Enhanced ERα transcriptional activity due to 14-3-3β increased the expressions of the endogenous ERα target genes, leading to proliferation of breast cancer cells. We suggest that 14-3-3β has oncogenic potential in breast cancer via binding to ERα and activation of the transcriptional activity of ERα.

AB - The estrogen receptor (ER) functions as a transcription factor that mediates the effects of estrogen. ERα, which plays a crucial role in the development and progression of breast cancer, is activated by estrogen binding, leading to receptor phosphorylation, dimerization, and recruitment of co-activators and chaperons to the estrogen-bound receptor complex. The 14-3-3 proteins bind to target proteins via phosphorylation and influence many cellular events by altering their subcellular localization or acting as a chaperone. However, regulation of ERα expression and transactivation by the 14-3-3 proteins has not been reported. We demonstrate that 14-3-3β functions as a positive regulator of ERα through a direct protein-protein interaction in an estrogen-dependent manner. Ectopic expression of 14-3-3β stimulated ERα-mediated transcriptional activity in MCF-7 breast cancer cells. Enhanced ERα transcriptional activity due to 14-3-3β increased the expressions of the endogenous ERα target genes, leading to proliferation of breast cancer cells. We suggest that 14-3-3β has oncogenic potential in breast cancer via binding to ERα and activation of the transcriptional activity of ERα.

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The role of 14-3-3β in transcriptional activation of estrogen receptor α and its involvement in proliferation of breast cancer cells

Abstract

Bibliographical note

UN SDGs

Keywords

ASJC Scopus subject areas

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