The sequence variation responsible for the functional difference between the CONSTANS protein, and the CONSTANS-like (COL) 1 and COL2 proteins, resides mostly in the region encoded by their first exons

Soon Kap Kim; Hyo Young Park; Yun Hee Jang; Jeong Hwan Lee; Jeong Kook Kim

doi:10.1016/j.plantsci.2012.09.019

The sequence variation responsible for the functional difference between the CONSTANS protein, and the CONSTANS-like (COL) 1 and COL2 proteins, resides mostly in the region encoded by their first exons

Soon Kap Kim, Hyo Young Park, Yun Hee Jang, Jeong Hwan Lee, Jeong Kook Kim

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Although the protein CONSTANS (CO) and its close relatives CONSTANS-like (COL) 1 and COL2 exhibit high amino acid sequence similarities, only the CO protein regulates floral induction in Arabidopsis. To investigate the structural basis for the functional differences between CO, COL1, and COL2 in flowering, we performed domain-swapping between CO, COL1, and COL2, and site-directed mutagenesis on the first exon of CO. The results suggest that the lack of flowering promotion activity by COL1 and COL2 is mainly attributed to the differences between CO and the COL1 and COL2 proteins in the amino acid sequence encoded by their first exons.

Original language	English
Pages (from-to)	71-78
Number of pages	8
Journal	Plant Science
Volume	199-200
DOIs	https://doi.org/10.1016/j.plantsci.2012.09.019
Publication status	Published - 2013 Feb

Bibliographical note

Funding Information:
We thank Prof. J.H. Ahn for the co-9 seeds. This work was supported by grants from the Plant Signaling Network Research Center , the Next-Generation BioGreen 21 Program ( PJ007978 ), Rural Development Administration, Republic of Korea (to J.-K. Kim), and the BK 21 program (to S.-K. Kim and J.H. Lee).

Keywords

CO
COL1
COL2
Domain swapping
Evolution
Flowering time

ASJC Scopus subject areas

Genetics
Agronomy and Crop Science
Plant Science

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10.1016/j.plantsci.2012.09.019

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The sequence variation responsible for the functional difference between the CONSTANS protein, and the CONSTANS-like (COL) 1 and COL2 proteins, resides mostly in the region encoded by their first exons. / Kim, Soon Kap; Park, Hyo Young; Jang, Yun Hee et al.
In: Plant Science, Vol. 199-200, 02.2013, p. 71-78.

Research output: Contribution to journal › Article › peer-review

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title = "The sequence variation responsible for the functional difference between the CONSTANS protein, and the CONSTANS-like (COL) 1 and COL2 proteins, resides mostly in the region encoded by their first exons",

abstract = "Although the protein CONSTANS (CO) and its close relatives CONSTANS-like (COL) 1 and COL2 exhibit high amino acid sequence similarities, only the CO protein regulates floral induction in Arabidopsis. To investigate the structural basis for the functional differences between CO, COL1, and COL2 in flowering, we performed domain-swapping between CO, COL1, and COL2, and site-directed mutagenesis on the first exon of CO. The results suggest that the lack of flowering promotion activity by COL1 and COL2 is mainly attributed to the differences between CO and the COL1 and COL2 proteins in the amino acid sequence encoded by their first exons.",

keywords = "CO, COL1, COL2, Domain swapping, Evolution, Flowering time",

author = "Kim, {Soon Kap} and Park, {Hyo Young} and Jang, {Yun Hee} and Lee, {Jeong Hwan} and Kim, {Jeong Kook}",

note = "Funding Information: We thank Prof. J.H. Ahn for the co-9 seeds. This work was supported by grants from the Plant Signaling Network Research Center , the Next-Generation BioGreen 21 Program ( PJ007978 ), Rural Development Administration, Republic of Korea (to J.-K. Kim), and the BK 21 program (to S.-K. Kim and J.H. Lee). ",

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T1 - The sequence variation responsible for the functional difference between the CONSTANS protein, and the CONSTANS-like (COL) 1 and COL2 proteins, resides mostly in the region encoded by their first exons

AU - Kim, Soon Kap

AU - Park, Hyo Young

AU - Jang, Yun Hee

AU - Lee, Jeong Hwan

AU - Kim, Jeong Kook

N1 - Funding Information: We thank Prof. J.H. Ahn for the co-9 seeds. This work was supported by grants from the Plant Signaling Network Research Center , the Next-Generation BioGreen 21 Program ( PJ007978 ), Rural Development Administration, Republic of Korea (to J.-K. Kim), and the BK 21 program (to S.-K. Kim and J.H. Lee).

PY - 2013/2

Y1 - 2013/2

N2 - Although the protein CONSTANS (CO) and its close relatives CONSTANS-like (COL) 1 and COL2 exhibit high amino acid sequence similarities, only the CO protein regulates floral induction in Arabidopsis. To investigate the structural basis for the functional differences between CO, COL1, and COL2 in flowering, we performed domain-swapping between CO, COL1, and COL2, and site-directed mutagenesis on the first exon of CO. The results suggest that the lack of flowering promotion activity by COL1 and COL2 is mainly attributed to the differences between CO and the COL1 and COL2 proteins in the amino acid sequence encoded by their first exons.

AB - Although the protein CONSTANS (CO) and its close relatives CONSTANS-like (COL) 1 and COL2 exhibit high amino acid sequence similarities, only the CO protein regulates floral induction in Arabidopsis. To investigate the structural basis for the functional differences between CO, COL1, and COL2 in flowering, we performed domain-swapping between CO, COL1, and COL2, and site-directed mutagenesis on the first exon of CO. The results suggest that the lack of flowering promotion activity by COL1 and COL2 is mainly attributed to the differences between CO and the COL1 and COL2 proteins in the amino acid sequence encoded by their first exons.

KW - CO

KW - COL1

KW - COL2

KW - Domain swapping

KW - Evolution

KW - Flowering time

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DO - 10.1016/j.plantsci.2012.09.019

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SN - 0168-9452

VL - 199-200

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EP - 78

JO - Plant Science

JF - Plant Science

ER -

The sequence variation responsible for the functional difference between the CONSTANS protein, and the CONSTANS-like (COL) 1 and COL2 proteins, resides mostly in the region encoded by their first exons

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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