The sequence variation responsible for the functional difference between the CONSTANS protein, and the CONSTANS-like (COL) 1 and COL2 proteins, resides mostly in the region encoded by their first exons

Soon Kap Kim, Hyo Young Park, Yun Hee Jang, Jeong Hwan Lee, Jeong Kook Kim

    Research output: Contribution to journalArticlepeer-review

    24 Citations (Scopus)

    Abstract

    Although the protein CONSTANS (CO) and its close relatives CONSTANS-like (COL) 1 and COL2 exhibit high amino acid sequence similarities, only the CO protein regulates floral induction in Arabidopsis. To investigate the structural basis for the functional differences between CO, COL1, and COL2 in flowering, we performed domain-swapping between CO, COL1, and COL2, and site-directed mutagenesis on the first exon of CO. The results suggest that the lack of flowering promotion activity by COL1 and COL2 is mainly attributed to the differences between CO and the COL1 and COL2 proteins in the amino acid sequence encoded by their first exons.

    Original languageEnglish
    Pages (from-to)71-78
    Number of pages8
    JournalPlant Science
    Volume199-200
    DOIs
    Publication statusPublished - 2013 Feb

    Bibliographical note

    Funding Information:
    We thank Prof. J.H. Ahn for the co-9 seeds. This work was supported by grants from the Plant Signaling Network Research Center , the Next-Generation BioGreen 21 Program ( PJ007978 ), Rural Development Administration, Republic of Korea (to J.-K. Kim), and the BK 21 program (to S.-K. Kim and J.H. Lee).

    Keywords

    • CO
    • COL1
    • COL2
    • Domain swapping
    • Evolution
    • Flowering time

    ASJC Scopus subject areas

    • Genetics
    • Agronomy and Crop Science
    • Plant Science

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