The SH3 Domain of Phospholipase C-γ1 Associates with Shc

Myung Jong Kim, Jong Ik Hwang, Jong Soo Chang, Sung Ho Ryu, Pann Ghill Suh

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


The SH3 domain of PLC-γ1 has been known to induce DNA synthesis. However, little is known about the putative effector proteins that associate with the domain. In this report, we provide evidence that the SH3 domain of PLC-γ1 associates with Shc, which has been implicated in the activation of p21Ras in response to many growth factors. The association between Shc and PLC-γ1 is enhanced either by ν-Src-induced transformation or EGF-stimulation in vivo and in vitro. Furthermore, from transient expression studies with COS-7 cells, we show that the SH3 domain of PLC-γ1 is required for association with Shc in vivo, whereas tyrosyl phosphorylation of PLC-γ1 is not. Taken together, we suggest that Shc might be involved in the PLC-γ1-mediated signaling pathway.

Original languageEnglish
Pages (from-to)119-126
Number of pages8
JournalJournal of Biochemistry and Molecular Biology
Issue number2
Publication statusPublished - 1999 Mar 31
Externally publishedYes


  • GST
  • PLC-γ1
  • Protein-Protein Interaction
  • SH3 domain
  • Shc

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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