The transcription factor γMYB2 acts as a negative regulator of secondary cell wall thickening in anther and stem

Ha Thi Kim Nguyen; Sujin Hyoung; Hae Jin Kim; Kwang Moon Cho; Jeong Sheop Shin

doi:10.1016/j.gene.2019.03.061

The transcription factor γMYB2 acts as a negative regulator of secondary cell wall thickening in anther and stem

Ha Thi Kim Nguyen, Sujin Hyoung, Hae Jin Kim, Kwang Moon Cho, Jeong Sheop Shin

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Secondary cell wall (SCW) thickening provides the mechanical force for anther dehiscence and plays an important role in the formation of xylem structure. We have previously reported that γMYB2, a MYB coiled-coil protein, directly binds to the P1BS cis-element of the PLA ₂ -γ promoter and acts as a co-activator of γMYB1 in controlling the expression of PLA ₂ -γ. In this study, we analyzed morphological phenotypes of the constitutive overexpression (γMYB2-OE) and knock-down (γMYB2-KD) lines of γMYB2. We found that γMYB2 overexpression caused the collapse of the endothecium layer, thereby suppressing anther dehiscence and forming short infertile siliques. The γMYB2-OE also showed less cellulose deposition in the xylem and had a longer primary stem than the wild-type, while γMYB2-KD had greater cellulose accumulation and a shorter primary stem than the wild-type. We demonstrated that the male sterility and the longer primary stem in γMYB2-OE were caused by reduced expression of SCW thickening-related genes. Our results suggest that γMYB2 acts as a negative regulator in controlling the SCW thickening in Arabidopsis.

Original language	English
Pages (from-to)	158-165
Number of pages	8
Journal	Gene
Volume	702
DOIs	https://doi.org/10.1016/j.gene.2019.03.061
Publication status	Published - 2019 Jun 20

Bibliographical note

Funding Information:
We would like to thank to Dr. Mitsuda (Bioproduction Research Institute, The National Institute of Advanced Industrial Science and Technology, JAPAN) for providing seeds of double knock-down mutant nst1nst2. This work was supported by a grant (NRF-2014R1A2A2A01005974) from the National Research Foundation of Korea (NRF) funded by the Ministry of Science and Technology and by a grant (PJ013670) from the Next-Generation BioGreen 21 Program funded by the Rural Development Administration, Republic of Korea. This work was also partially supported by Korea University.

Funding Information:
We would like to thank to Dr. Mitsuda (Bioproduction Research Institute, The National Institute of Advanced Industrial Science and Technology, JAPAN) for providing seeds of double knock-down mutant nst1nst2. This work was supported by a grant ( NRF-2014R1A2A2A01005974 ) from the National Research Foundation of Korea (NRF) funded by the Ministry of Science and Technology and by a grant ( PJ013670 ) from the Next-Generation BioGreen 21 Program funded by the Rural Development Administration , Republic of Korea. This work was also partially supported by Korea University .

Keywords

Anther dehiscence
Arabidopsis thaliana
Endothecium
Negative regulator
Secondary cell wall
γMYB2

ASJC Scopus subject areas

Genetics

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10.1016/j.gene.2019.03.061

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@article{420b63aa805547fb81353519ffab2717,

title = "The transcription factor γMYB2 acts as a negative regulator of secondary cell wall thickening in anther and stem",

abstract = " Secondary cell wall (SCW) thickening provides the mechanical force for anther dehiscence and plays an important role in the formation of xylem structure. We have previously reported that γMYB2, a MYB coiled-coil protein, directly binds to the P1BS cis-element of the PLA 2 -γ promoter and acts as a co-activator of γMYB1 in controlling the expression of PLA 2 -γ. In this study, we analyzed morphological phenotypes of the constitutive overexpression (γMYB2-OE) and knock-down (γMYB2-KD) lines of γMYB2. We found that γMYB2 overexpression caused the collapse of the endothecium layer, thereby suppressing anther dehiscence and forming short infertile siliques. The γMYB2-OE also showed less cellulose deposition in the xylem and had a longer primary stem than the wild-type, while γMYB2-KD had greater cellulose accumulation and a shorter primary stem than the wild-type. We demonstrated that the male sterility and the longer primary stem in γMYB2-OE were caused by reduced expression of SCW thickening-related genes. Our results suggest that γMYB2 acts as a negative regulator in controlling the SCW thickening in Arabidopsis. ",

keywords = "Anther dehiscence, Arabidopsis thaliana, Endothecium, Negative regulator, Secondary cell wall, γMYB2",

author = "Nguyen, {Ha Thi Kim} and Sujin Hyoung and Kim, {Hae Jin} and Cho, {Kwang Moon} and Shin, {Jeong Sheop}",

note = "Funding Information: We would like to thank to Dr. Mitsuda (Bioproduction Research Institute, The National Institute of Advanced Industrial Science and Technology, JAPAN) for providing seeds of double knock-down mutant nst1nst2. This work was supported by a grant (NRF-2014R1A2A2A01005974) from the National Research Foundation of Korea (NRF) funded by the Ministry of Science and Technology and by a grant (PJ013670) from the Next-Generation BioGreen 21 Program funded by the Rural Development Administration, Republic of Korea. This work was also partially supported by Korea University. Funding Information: We would like to thank to Dr. Mitsuda (Bioproduction Research Institute, The National Institute of Advanced Industrial Science and Technology, JAPAN) for providing seeds of double knock-down mutant nst1nst2. This work was supported by a grant ( NRF-2014R1A2A2A01005974 ) from the National Research Foundation of Korea (NRF) funded by the Ministry of Science and Technology and by a grant ( PJ013670 ) from the Next-Generation BioGreen 21 Program funded by the Rural Development Administration , Republic of Korea. This work was also partially supported by Korea University . ",

year = "2019",

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doi = "10.1016/j.gene.2019.03.061",

language = "English",

volume = "702",

pages = "158--165",

journal = "Gene",

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T1 - The transcription factor γMYB2 acts as a negative regulator of secondary cell wall thickening in anther and stem

AU - Nguyen, Ha Thi Kim

AU - Hyoung, Sujin

AU - Kim, Hae Jin

AU - Cho, Kwang Moon

AU - Shin, Jeong Sheop

N1 - Funding Information: We would like to thank to Dr. Mitsuda (Bioproduction Research Institute, The National Institute of Advanced Industrial Science and Technology, JAPAN) for providing seeds of double knock-down mutant nst1nst2. This work was supported by a grant (NRF-2014R1A2A2A01005974) from the National Research Foundation of Korea (NRF) funded by the Ministry of Science and Technology and by a grant (PJ013670) from the Next-Generation BioGreen 21 Program funded by the Rural Development Administration, Republic of Korea. This work was also partially supported by Korea University. Funding Information: We would like to thank to Dr. Mitsuda (Bioproduction Research Institute, The National Institute of Advanced Industrial Science and Technology, JAPAN) for providing seeds of double knock-down mutant nst1nst2. This work was supported by a grant ( NRF-2014R1A2A2A01005974 ) from the National Research Foundation of Korea (NRF) funded by the Ministry of Science and Technology and by a grant ( PJ013670 ) from the Next-Generation BioGreen 21 Program funded by the Rural Development Administration , Republic of Korea. This work was also partially supported by Korea University .

PY - 2019/6/20

Y1 - 2019/6/20

N2 - Secondary cell wall (SCW) thickening provides the mechanical force for anther dehiscence and plays an important role in the formation of xylem structure. We have previously reported that γMYB2, a MYB coiled-coil protein, directly binds to the P1BS cis-element of the PLA 2 -γ promoter and acts as a co-activator of γMYB1 in controlling the expression of PLA 2 -γ. In this study, we analyzed morphological phenotypes of the constitutive overexpression (γMYB2-OE) and knock-down (γMYB2-KD) lines of γMYB2. We found that γMYB2 overexpression caused the collapse of the endothecium layer, thereby suppressing anther dehiscence and forming short infertile siliques. The γMYB2-OE also showed less cellulose deposition in the xylem and had a longer primary stem than the wild-type, while γMYB2-KD had greater cellulose accumulation and a shorter primary stem than the wild-type. We demonstrated that the male sterility and the longer primary stem in γMYB2-OE were caused by reduced expression of SCW thickening-related genes. Our results suggest that γMYB2 acts as a negative regulator in controlling the SCW thickening in Arabidopsis.

AB - Secondary cell wall (SCW) thickening provides the mechanical force for anther dehiscence and plays an important role in the formation of xylem structure. We have previously reported that γMYB2, a MYB coiled-coil protein, directly binds to the P1BS cis-element of the PLA 2 -γ promoter and acts as a co-activator of γMYB1 in controlling the expression of PLA 2 -γ. In this study, we analyzed morphological phenotypes of the constitutive overexpression (γMYB2-OE) and knock-down (γMYB2-KD) lines of γMYB2. We found that γMYB2 overexpression caused the collapse of the endothecium layer, thereby suppressing anther dehiscence and forming short infertile siliques. The γMYB2-OE also showed less cellulose deposition in the xylem and had a longer primary stem than the wild-type, while γMYB2-KD had greater cellulose accumulation and a shorter primary stem than the wild-type. We demonstrated that the male sterility and the longer primary stem in γMYB2-OE were caused by reduced expression of SCW thickening-related genes. Our results suggest that γMYB2 acts as a negative regulator in controlling the SCW thickening in Arabidopsis.

KW - Anther dehiscence

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KW - Endothecium

KW - Negative regulator

KW - Secondary cell wall

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SN - 0378-1119

VL - 702

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JO - Gene

JF - Gene

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The transcription factor γMYB2 acts as a negative regulator of secondary cell wall thickening in anther and stem

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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