The transcriptional co-activator TAZ interacts differentially with transcriptional enhancer factor-1 (TEF-1) family members

William M. Mahoney, Jeong Ho Hong, Michael B. Yaffe, Iain K.G. Farrance

Research output: Contribution to journalArticlepeer-review

182 Citations (Scopus)


Members of the highly related TEF-1 (transcriptional enhancer factor-1) family (also known as TEAD, for TEF-1, TEC1, ABAA domain) bind to MCAT (muscle C, A and T sites) and A/T-rich sites in promoters active in cardiac, skeletal and smooth muscle, placenta, and neural crest. TEF-1 activity is regulated by interactions with transcriptional co-factors [p160, TONDU (Vgl-1, Vestigial-like protein-1), Vgl-2 and YAP65 (Yes-associated protein 63 kDa)]. The strong transcriptional co-activator YAP65 interacts with all TEF-1 family members, and, since YAP65 is related to TAZ (transcriptional co-activator with PDZ-binding motif), we wanted to determine if TAZ also interacts with members of the TEF-1 family. In the present study, we show by GST (glutathione S-transferase) pull-down assays, by co-immunoprecipitation and by modified mammalian two-hybrid assays that TEF-1 interacts with TAZ in vitro and in vivo. Electrophoretic mobility-shift assays with purified TEF-1 and GST-TAZ fusion protein showed that TAZ interacts with TEF-1 bound to MCAT DNA, TAZ can interact with endogenous TEF-1 proteins, since exogenous TAZ activated MCAT-dependent reporter promoters. Like YAP65, TAZ interacted with all four TEF-1 family members. GST pull-down assays with increasing amounts of [35S]TEF-1 and [ 35S]RTEF-1 (related TEF-1) showed that TAZ interacts more efficiently with TEF-1 than with RTEF-1. This differential interaction also extended to the interaction of TEF-1 and RTEF-1 with TAZ in vivo, as assayed by a modified mammalian two-hybrid experiment. These data show that differential association of TEF-1 proteins with transcriptional co-activators may regulate the activity of TEF-1 family members.

Original languageEnglish
Pages (from-to)217-225
Number of pages9
JournalBiochemical Journal
Issue number1
Publication statusPublished - 2005 May 15
Externally publishedYes


  • ABAA domain (TEAD)
  • Muscle
  • TEC1
  • TEF-1
  • Transcriptional co-activator
  • Transcriptional co-activator with PDZ-binding motif (TAZ)
  • Transcriptional enhancer factor-1 (TEF-1)
  • Yes-associated protein 65 kDa (YAP65)

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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