The type III calcium/calmodulin-sensitive adenylyl cyclase is not specific to olfactory sensory neurons

Zhengui Xia; Eui Ju Choi; Fan Wang; Daniel R. Storm

doi:10.1016/0304-3940(92)90742-P

The type III calcium/calmodulin-sensitive adenylyl cyclase is not specific to olfactory sensory neurons

Zhengui Xia
, Eui Ju Choi
, Fan Wang
, Daniel R. Storm^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

83 Citations (Scopus)

Abstract

A cDNA clone for type III adenylyl cyclase was originally isolated from a rat olfactory cDNA library and Northern analysis using total RNA suggested that the expression of the type III mRNA may be limited to the olfactory epithelium (Bakalyar and Reed, Science, 250 (1990) 1403-1406). In this study, the distribution of type III adenylyl cyclase mRNA in a number of bovine tissues and cultured cells was examined by Northern analysis using poly(A)⁺ RNA. Type III adenylyl cyclase mRNA was expressed in brain, spinal cord, adrenal medulla, adrenal cortex, heart atrium, aorta, lung, retina, 293 cells and PC-12 cells. Furthermore, the Ca²⁺ sensitivity of adenylyl cyclase activity in 293 cells indicated the presence of type III adenylyl cyclase. These data indicate that expression of the type III adenylyl cyclase is not limited to olfactory tissues, and that this enzyme probably has a number of physiological functions in addition to olfactory signal transduction.

Original language	English
Pages (from-to)	169-173
Number of pages	5
Journal	Neuroscience Letters
Volume	144
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3940(92)90742-P
Publication status	Published - 1992 Sept 14
Externally published	Yes

Bibliographical note

Funding Information:
We would like to thank Dr R. R. Reed (Johns Hopkins School of Medicine) for kindly providing the rat type IlI adenylyl cyclase cDNA. This research was supported by NIH Grants NS 20498 and HL 44948. Z.X. and EJ.C. were supported by Washington Heart Association postdoctoral fellowships.

Keywords

Ca
Calmodulin
Tissue distribution
Type III adenylyl cyclase

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(92)90742-P

Cite this

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title = "The type III calcium/calmodulin-sensitive adenylyl cyclase is not specific to olfactory sensory neurons",

abstract = "A cDNA clone for type III adenylyl cyclase was originally isolated from a rat olfactory cDNA library and Northern analysis using total RNA suggested that the expression of the type III mRNA may be limited to the olfactory epithelium (Bakalyar and Reed, Science, 250 (1990) 1403-1406). In this study, the distribution of type III adenylyl cyclase mRNA in a number of bovine tissues and cultured cells was examined by Northern analysis using poly(A)+ RNA. Type III adenylyl cyclase mRNA was expressed in brain, spinal cord, adrenal medulla, adrenal cortex, heart atrium, aorta, lung, retina, 293 cells and PC-12 cells. Furthermore, the Ca2+ sensitivity of adenylyl cyclase activity in 293 cells indicated the presence of type III adenylyl cyclase. These data indicate that expression of the type III adenylyl cyclase is not limited to olfactory tissues, and that this enzyme probably has a number of physiological functions in addition to olfactory signal transduction.",

keywords = "Ca, Calmodulin, Tissue distribution, Type III adenylyl cyclase",

author = "Zhengui Xia and Choi, \{Eui Ju\} and Fan Wang and Storm, \{Daniel R.\}",

note = "Funding Information: We would like to thank Dr R. R. Reed (Johns Hopkins School of Medicine) for kindly providing the rat type IlI adenylyl cyclase cDNA. This research was supported by NIH Grants NS 20498 and HL 44948. Z.X. and EJ.C. were supported by Washington Heart Association postdoctoral fellowships.",

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doi = "10.1016/0304-3940(92)90742-P",

language = "English",

volume = "144",

pages = "169--173",

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AU - Xia, Zhengui

AU - Choi, Eui Ju

AU - Wang, Fan

AU - Storm, Daniel R.

N1 - Funding Information: We would like to thank Dr R. R. Reed (Johns Hopkins School of Medicine) for kindly providing the rat type IlI adenylyl cyclase cDNA. This research was supported by NIH Grants NS 20498 and HL 44948. Z.X. and EJ.C. were supported by Washington Heart Association postdoctoral fellowships.

PY - 1992/9/14

Y1 - 1992/9/14

N2 - A cDNA clone for type III adenylyl cyclase was originally isolated from a rat olfactory cDNA library and Northern analysis using total RNA suggested that the expression of the type III mRNA may be limited to the olfactory epithelium (Bakalyar and Reed, Science, 250 (1990) 1403-1406). In this study, the distribution of type III adenylyl cyclase mRNA in a number of bovine tissues and cultured cells was examined by Northern analysis using poly(A)+ RNA. Type III adenylyl cyclase mRNA was expressed in brain, spinal cord, adrenal medulla, adrenal cortex, heart atrium, aorta, lung, retina, 293 cells and PC-12 cells. Furthermore, the Ca2+ sensitivity of adenylyl cyclase activity in 293 cells indicated the presence of type III adenylyl cyclase. These data indicate that expression of the type III adenylyl cyclase is not limited to olfactory tissues, and that this enzyme probably has a number of physiological functions in addition to olfactory signal transduction.

AB - A cDNA clone for type III adenylyl cyclase was originally isolated from a rat olfactory cDNA library and Northern analysis using total RNA suggested that the expression of the type III mRNA may be limited to the olfactory epithelium (Bakalyar and Reed, Science, 250 (1990) 1403-1406). In this study, the distribution of type III adenylyl cyclase mRNA in a number of bovine tissues and cultured cells was examined by Northern analysis using poly(A)+ RNA. Type III adenylyl cyclase mRNA was expressed in brain, spinal cord, adrenal medulla, adrenal cortex, heart atrium, aorta, lung, retina, 293 cells and PC-12 cells. Furthermore, the Ca2+ sensitivity of adenylyl cyclase activity in 293 cells indicated the presence of type III adenylyl cyclase. These data indicate that expression of the type III adenylyl cyclase is not limited to olfactory tissues, and that this enzyme probably has a number of physiological functions in addition to olfactory signal transduction.

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KW - Calmodulin

KW - Tissue distribution

KW - Type III adenylyl cyclase

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IS - 1-2

ER -

The type III calcium/calmodulin-sensitive adenylyl cyclase is not specific to olfactory sensory neurons

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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