The wheat TaF-box3, SCF ubiquitin ligase component, participates in the regulation of flowering time in transgenic Arabidopsis

Jae Ho Kim, Woo Joo Jung, Moon Seok Kim, Yong Weon Seo

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Histone methylation is actively involved in plant flowering time and is regulated by a myriad of genetic pathways that integrate endogenous and exogenous signals. We identified an F-box gene from wheat (Triticum aestivum L.) and named it TaF-box3. Transcript expression analysis showed that TaF-box3 expression was gradually induced during the floret development and anthesis stages (WS2.5–10). Furthermore, ubiquitination assays have shown that TaF-box3 is a key component of the SCF ubiquitin ligase complex. TaF-box3 overexpression in Arabidopsis resulted in an early flowering phenotype and different cell sizes in leaves compared to the WT. Furthermore, the transcript level of a flowering time-related gene was significantly reduced in TaF-box3 overexpressing plants, which was linked with lower histone H3 Lys4 trimethylation (H3K4me3) and H3 Lys36 trimethylation (H3K36me3). Overexpression of TaF-box3 in Arabidopsis was shown to be involved in the regulation of flowering time by demethylating FLC chromatin, according to ChIP experiments. Protein analysis confirmed that TaMETS interacts with TaF-box3 and is ubiquitinated and degraded in a TaF-box3-dependnent manner. Based on these findings, we propose that TaF-box3 has a positive role in flowering time, which leads to a better understanding of TaF-box3 physiological mechanism in Arabidopsis.

Original languageEnglish
Article number111668
JournalPlant Science
Volume331
DOIs
Publication statusPublished - 2023 Jun

Bibliographical note

Funding Information:
This work was supported by a grant from the Korea University and Basic Science Research Program through the National Research Foundation of Korea , which is funded by the Ministry of Science, ICT & Future Planning [grant number 2017R1D1A1B06030349 ].

Publisher Copyright:
© 2023 Elsevier B.V.

Keywords

  • Arabidopsis
  • Flowering time
  • SCF ubiquitin ligase
  • TaF-box3
  • Wheat

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

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