Thermal and operational characteristics of glutaryl-7-aminocephalosporanic acid acylase immobilized on silica gel modified by epoxide silanization

Jung Soo Lim; Seung Won Park; Seung Wook Kim

doi:10.1007/s11274-005-5744-5

Thermal and operational characteristics of glutaryl-7-aminocephalosporanic acid acylase immobilized on silica gel modified by epoxide silanization

Jung Soo Lim, Seung Won Park, Seung Wook Kim

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

In this study, an investigation was performed into the thermal and operational characteristics of glutaryl-7-aminocephalosporanic acid (GL-7-ACA) acylase (EC 3.5.1.-) immobilized on silica gel that had been modified by epoxide silanization. The pH values for the optimum activity of free and immobilized GL-7-ACA acylase were almost the same. However, the pH-dependent activity profile for the immobilized GL-7-ACA acylase is considerably expanded. Both free and immobilized enzymes generally had the highest activity at 50°C. In thermodynamic studies, it was found that immobilization using epoxide silanization made GL-7-ACA acylase thermodynamically stable. In the results of repeated batch production of 7-ACA, 89.0 and 83.5% of the 7-ACA produced at the initial cycle were maintained after 20 times of recycle at 25°C and 30°C, respectively. Hence it was suggested that mass production of 7-ACA at 25°C using immobilized GL-7-ACA acylase by epoxide silanization would be possible on a large scale.

Original language	English
Pages (from-to)	39-44
Number of pages	6
Journal	World Journal of Microbiology and Biotechnology
Volume	22
Issue number	1
DOIs	https://doi.org/10.1007/s11274-005-5744-5
Publication status	Published - 2006 Jan

Bibliographical note

Funding Information:
This study was supported by research grants from the Korea Science and Engineering Foundation (KOSEF) through the Applied Rheology Center (ARC), an official KOSEF-created engineering research center (ERC) at Korea University, Seoul, Korea.

Keywords

Epoxide silanization
GL-7-ACA Acylase
Immobilization
Repeated batch reaction
Thermodynamics

ASJC Scopus subject areas

Biotechnology
Physiology
Applied Microbiology and Biotechnology

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10.1007/s11274-005-5744-5

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title = "Thermal and operational characteristics of glutaryl-7-aminocephalosporanic acid acylase immobilized on silica gel modified by epoxide silanization",

abstract = "In this study, an investigation was performed into the thermal and operational characteristics of glutaryl-7-aminocephalosporanic acid (GL-7-ACA) acylase (EC 3.5.1.-) immobilized on silica gel that had been modified by epoxide silanization. The pH values for the optimum activity of free and immobilized GL-7-ACA acylase were almost the same. However, the pH-dependent activity profile for the immobilized GL-7-ACA acylase is considerably expanded. Both free and immobilized enzymes generally had the highest activity at 50°C. In thermodynamic studies, it was found that immobilization using epoxide silanization made GL-7-ACA acylase thermodynamically stable. In the results of repeated batch production of 7-ACA, 89.0 and 83.5% of the 7-ACA produced at the initial cycle were maintained after 20 times of recycle at 25°C and 30°C, respectively. Hence it was suggested that mass production of 7-ACA at 25°C using immobilized GL-7-ACA acylase by epoxide silanization would be possible on a large scale.",

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author = "Lim, {Jung Soo} and Park, {Seung Won} and Kim, {Seung Wook}",

note = "Funding Information: This study was supported by research grants from the Korea Science and Engineering Foundation (KOSEF) through the Applied Rheology Center (ARC), an official KOSEF-created engineering research center (ERC) at Korea University, Seoul, Korea.",

year = "2006",

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doi = "10.1007/s11274-005-5744-5",

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AU - Kim, Seung Wook

N1 - Funding Information: This study was supported by research grants from the Korea Science and Engineering Foundation (KOSEF) through the Applied Rheology Center (ARC), an official KOSEF-created engineering research center (ERC) at Korea University, Seoul, Korea.

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N2 - In this study, an investigation was performed into the thermal and operational characteristics of glutaryl-7-aminocephalosporanic acid (GL-7-ACA) acylase (EC 3.5.1.-) immobilized on silica gel that had been modified by epoxide silanization. The pH values for the optimum activity of free and immobilized GL-7-ACA acylase were almost the same. However, the pH-dependent activity profile for the immobilized GL-7-ACA acylase is considerably expanded. Both free and immobilized enzymes generally had the highest activity at 50°C. In thermodynamic studies, it was found that immobilization using epoxide silanization made GL-7-ACA acylase thermodynamically stable. In the results of repeated batch production of 7-ACA, 89.0 and 83.5% of the 7-ACA produced at the initial cycle were maintained after 20 times of recycle at 25°C and 30°C, respectively. Hence it was suggested that mass production of 7-ACA at 25°C using immobilized GL-7-ACA acylase by epoxide silanization would be possible on a large scale.

AB - In this study, an investigation was performed into the thermal and operational characteristics of glutaryl-7-aminocephalosporanic acid (GL-7-ACA) acylase (EC 3.5.1.-) immobilized on silica gel that had been modified by epoxide silanization. The pH values for the optimum activity of free and immobilized GL-7-ACA acylase were almost the same. However, the pH-dependent activity profile for the immobilized GL-7-ACA acylase is considerably expanded. Both free and immobilized enzymes generally had the highest activity at 50°C. In thermodynamic studies, it was found that immobilization using epoxide silanization made GL-7-ACA acylase thermodynamically stable. In the results of repeated batch production of 7-ACA, 89.0 and 83.5% of the 7-ACA produced at the initial cycle were maintained after 20 times of recycle at 25°C and 30°C, respectively. Hence it was suggested that mass production of 7-ACA at 25°C using immobilized GL-7-ACA acylase by epoxide silanization would be possible on a large scale.

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Thermal and operational characteristics of glutaryl-7-aminocephalosporanic acid acylase immobilized on silica gel modified by epoxide silanization

Abstract

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Keywords

ASJC Scopus subject areas

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