Thermodynamically controlled separation of polyvalent 2-nm gold nanoparticle-oligonucleotide conjugates

Jae Seung Lee, Dwight S. Seferos, David A. Giljohann, Chad A. Mirkin

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)


We describe the synthesis of small (2-nm diameter) gold nanoparticles densely functionalized with thiolated DNA (DNA-Au NPs) and a method to separate these particles from excess free DNA after synthesis. The separation method utilizes the thermodynamically enhanced binding properties of 2-nm DNA-Au NPs, compared to free excess DNA, to selectively hybridize these small particles to larger (15-nm diameter) DNA-Au NPs and form aggregates that can be isolated by simple centrifugation. These 2-nm DNA-Au NPs are obtained in a 46% overall yield, have a high surface coverage of DNA (64.8 ± 6.4 pmol/cm2), and as a result, exhibit increased melting temperatures and cooperative melting properties.

Original languageEnglish
Pages (from-to)5430-5431
Number of pages2
JournalJournal of the American Chemical Society
Issue number16
Publication statusPublished - 2008 Apr 23

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry
  • Biochemistry
  • Colloid and Surface Chemistry


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