Thermophilic L-fucose isomerase from Thermanaeromonas toyohensis for L-fucose synthesis from L-fuculose

In Jung Kim, Kyoung Heon Kim

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The beneficial biological properties of L-fucose have extended its commercial application potential in pharmaceutical, cosmetic, and food industries. Enzymatic production of L-fucose with L-fucose isomerase (L-FucI) is considered a selective, green, and efficient strategy. Efficient sugar production requires thermophilic enzymes with increased reaction rate, reduced risk of microbial contamination, and high sugar solubility. No study has evaluated the applicability of thermophilic L-FucI for L-fucose production. In this study, we explored the biochemical properties of a thermostable L-FucI from Thermanaeromonas toyohensis (TtFucI) using L-fuculose as a substrate. The recombinant TtFucI exhibited thermophilicity and optimum activity at 70 °C. The specific activity, Km, and kcat toward L-fuculose were 199.8 U/mg, 33.4 mM, and 901.7 s−1, respectively. Mn2+ ions increased the activity of the enzyme by ∼10 times and enhanced its thermal stability. Our study, on L-fucose synthesis by thermostable L-FucI, suggests the potential application of this enzyme for the industrial production of L-fucose.

Original languageEnglish
Pages (from-to)131-137
Number of pages7
JournalProcess Biochemistry
Volume96
DOIs
Publication statusPublished - 2020 Sept

Bibliographical note

Funding Information:
This research was supported by the Mid-career Researcher Program (2020R1A2B5B02002631) and the Basic Research Laboratory Program (2018R1A4A1022589), both funded by the Korean Government (MSIT), through the National Research Foundation (NRF) of Korea. IJK acknowledges grant support from the Research Fellow Program through NRF (2017R1A6A3A11030496). Experiments were performed using the facilities of the Institute of Biomedical Science and Food Safety at the Food Safety Hall, Korea University.

Funding Information:
This research was supported by the Mid-career Researcher Program ( 2020R1A2B5B02002631 ) and the Basic Research Laboratory Program ( 2018R1A4A1022589 ), both funded by the Korean Government (MSIT) , through the National Research Foundation (NRF) of Korea . IJK acknowledges grant support from the Research Fellow Program through NRF ( 2017R1A6A3A11030496 ). Experiments were performed using the facilities of the Institute of Biomedical Science and Food Safety at the Food Safety Hall, Korea University.

Publisher Copyright:
© 2020

Keywords

  • L-
  • L-Fucose
  • L-fucose isomerase
  • Thermanaeromonas toyohensis
  • Thermophilic

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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