Thimerosal stimulates focal adhesion kinase and cytoskeletal changes by redox modulation

Euikyung Kim; Jung Hwan Kim; Hyeon Soo Kim; Sung Ho Ryu; Pann Ghill Suh

doi:10.1016/S0167-4889(02)00275-6

Thimerosal stimulates focal adhesion kinase and cytoskeletal changes by redox modulation

Euikyung Kim, Jung Hwan Kim, Hyeon Soo Kim, Sung Ho Ryu, Pann Ghill Suh

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Thimerosal is one of the most widely used preservatives and has been reported to cause chemically mediated side effects. However, the mechanism of the side effects is not clearly understood yet. In the present study, we showed that HeLa S cells treated by thimerosal generated reactive oxygen species (ROS). Thimerosal-generated ROS stimulated the tyrosine phosphorylation of focal adhesion kinase (FAK) and also induced cytoskeletal changes. Pretreatment with intracellular calcium chelator, BAPTA did not block the thimerosal-mediated FAK tyrosine phosphorylation. On the other hand, either FAK inhibitor, tyrphostin or ROS scavenger, N-acetyl-L-cysteine (NAC) suppressed the tyrosine phosphorylation and cytoskeletal changes. These results suggest that thimerosal seems to induce FAK tyrosine phosphorylation and cytoskeletal changes by ROS generation but not by intracellular calcium mobilization. We think the present finding can be an important clue to understanding the mechanism of thimerosal-mediated side effects, such as contact dermatitis, and allergy.

Original language	English
Pages (from-to)	9-15
Number of pages	7
Journal	Biochimica et Biophysica Acta - Molecular Cell Research
Volume	1593
Issue number	1
DOIs	https://doi.org/10.1016/S0167-4889(02)00275-6
Publication status	Published - 2002 Dec 16
Externally published	Yes

Keywords

Cytoskeletal change
Focal adhesion kinase
Reactive oxygen species
Thimerosal
Tyrosine phosphorylation

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/S0167-4889(02)00275-6

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title = "Thimerosal stimulates focal adhesion kinase and cytoskeletal changes by redox modulation",

abstract = "Thimerosal is one of the most widely used preservatives and has been reported to cause chemically mediated side effects. However, the mechanism of the side effects is not clearly understood yet. In the present study, we showed that HeLa S cells treated by thimerosal generated reactive oxygen species (ROS). Thimerosal-generated ROS stimulated the tyrosine phosphorylation of focal adhesion kinase (FAK) and also induced cytoskeletal changes. Pretreatment with intracellular calcium chelator, BAPTA did not block the thimerosal-mediated FAK tyrosine phosphorylation. On the other hand, either FAK inhibitor, tyrphostin or ROS scavenger, N-acetyl-L-cysteine (NAC) suppressed the tyrosine phosphorylation and cytoskeletal changes. These results suggest that thimerosal seems to induce FAK tyrosine phosphorylation and cytoskeletal changes by ROS generation but not by intracellular calcium mobilization. We think the present finding can be an important clue to understanding the mechanism of thimerosal-mediated side effects, such as contact dermatitis, and allergy.",

keywords = "Cytoskeletal change, Focal adhesion kinase, Reactive oxygen species, Thimerosal, Tyrosine phosphorylation",

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AU - Kim, Euikyung

AU - Kim, Jung Hwan

AU - Kim, Hyeon Soo

AU - Ryu, Sung Ho

AU - Suh, Pann Ghill

PY - 2002/12/16

Y1 - 2002/12/16

N2 - Thimerosal is one of the most widely used preservatives and has been reported to cause chemically mediated side effects. However, the mechanism of the side effects is not clearly understood yet. In the present study, we showed that HeLa S cells treated by thimerosal generated reactive oxygen species (ROS). Thimerosal-generated ROS stimulated the tyrosine phosphorylation of focal adhesion kinase (FAK) and also induced cytoskeletal changes. Pretreatment with intracellular calcium chelator, BAPTA did not block the thimerosal-mediated FAK tyrosine phosphorylation. On the other hand, either FAK inhibitor, tyrphostin or ROS scavenger, N-acetyl-L-cysteine (NAC) suppressed the tyrosine phosphorylation and cytoskeletal changes. These results suggest that thimerosal seems to induce FAK tyrosine phosphorylation and cytoskeletal changes by ROS generation but not by intracellular calcium mobilization. We think the present finding can be an important clue to understanding the mechanism of thimerosal-mediated side effects, such as contact dermatitis, and allergy.

AB - Thimerosal is one of the most widely used preservatives and has been reported to cause chemically mediated side effects. However, the mechanism of the side effects is not clearly understood yet. In the present study, we showed that HeLa S cells treated by thimerosal generated reactive oxygen species (ROS). Thimerosal-generated ROS stimulated the tyrosine phosphorylation of focal adhesion kinase (FAK) and also induced cytoskeletal changes. Pretreatment with intracellular calcium chelator, BAPTA did not block the thimerosal-mediated FAK tyrosine phosphorylation. On the other hand, either FAK inhibitor, tyrphostin or ROS scavenger, N-acetyl-L-cysteine (NAC) suppressed the tyrosine phosphorylation and cytoskeletal changes. These results suggest that thimerosal seems to induce FAK tyrosine phosphorylation and cytoskeletal changes by ROS generation but not by intracellular calcium mobilization. We think the present finding can be an important clue to understanding the mechanism of thimerosal-mediated side effects, such as contact dermatitis, and allergy.

KW - Cytoskeletal change

KW - Focal adhesion kinase

KW - Reactive oxygen species

KW - Thimerosal

KW - Tyrosine phosphorylation

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Thimerosal stimulates focal adhesion kinase and cytoskeletal changes by redox modulation

Abstract

Keywords

ASJC Scopus subject areas

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