Three-dimensional bioprinting of rat embryonic neural cells

Wonhye Lee, Jason Pinckney, Vivian Lee, Jong Hwan Lee, Krisztina Fischer, Samuel Polio, Je Kyun Park, Seung Schik Yoo

Research output: Contribution to journalArticlepeer-review

137 Citations (Scopus)


We present a direct cell printing technique to pattern neural cells in a three-dimensional (3D) multilayered collagen gel. A layer of collagen precursor was printed to provide a scaffold for the cells, and the rat embryonic neurons and astrocytes were subsequently printed on the layer. A solution of sodium bicarbonate was applied to the cell containing collagen layer as nebulized aerosols, which allowed the gelation of the collagen. This process was repeated layer-by-layer to construct the 3D cell-hydrogel composites. Upon characterizing the relationship between printing resolutions and the growth of printed neural cells, single/multiple layers of neural cell-hydrogel composites were constructed and cultured. The on-demand capability to print neural cells in a multilayered hydrogel scaffold offers flexibility in generating artificial 3D neural tissue composites.

Original languageEnglish
Pages (from-to)798-803
Number of pages6
Issue number8
Publication statusPublished - 2009 May 27
Externally publishedYes


  • Astrocytes
  • Cell printing
  • Collagen hydrogel
  • Freeform fabrication
  • Neurons
  • Tissue engineering

ASJC Scopus subject areas

  • General Neuroscience


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