Abstract
We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image for arbitrary shearing direction and bias retardation. In addition, we obtain images at different depths without a scanning objective lens by numerically propagating the acquired coherent images. Our method achieves high-resolution and high-contrast 3-D DIC imaging of live biological cells. The proposed method will be useful for monitoring 3-D dynamics of intracellular particles.
| Original language | English |
|---|---|
| Article number | 026003 |
| Journal | Journal of biomedical optics |
| Volume | 17 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2012 Feb |
Bibliographical note
Funding Information:This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (grant numbers 2011-0005018 and 2011-0016568), the National R&D Program for Cancer Control, the Ministry of Health & Welfare, the Republic of Korea (1120290), the IT Research and Development Program of MKE/KEIT (KI001810039169), the National Center for Research Resources of the National Institutes of Health (P41-RR02594-24), and the National Science Foundation (DBI-0754339).
Keywords
- image processing
- interferometry
- microscopy
- synthetic apertures
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Biomedical Engineering