Three-dimensional imaging for the analysis of human epidermal melanocytes

Dai Hyun Kim; Se Jeong Lee; Soo-Hong Seo; Hyo Hyun Ahn; Byung Jo Kim; Woong Sun; Im Joo Rhyu

doi:10.1111/pcmr.13051

Three-dimensional imaging for the analysis of human epidermal melanocytes

Dai Hyun Kim, Se Jeong Lee, Soo-Hong Seo, Hyo Hyun Ahn, Byung Jo Kim, Woong Sun, Im Joo Rhyu

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Three-dimensional (3-D) analysis of human epidermal melanocytes is required for deeper understanding of melanocytic disorders. The purpose of this study was to standardize 3-D imaging and quantification for the evaluation of epidermal melanocytes. The epidermal specimen was obtained using the suction blister method from a patient with melanocytic nevus on the forearm skin. Cutaneous ACT-PRESTO, the tissue-clearing and labeling technique, was subsequently performed. With the 3-D image analysis program, morphological reconstruction and quantification of selected perilesional and melanocytic nevus areas were possible. The region of melanocytic nevus showed higher numbers of total melanocytic dendrites and similar numbers of cell bodies compared with perilesional area. In addition, the mean area and volume of cell bodies increased in the melanocytic nevus area compared with the results in the perilesional area. The 3-D evaluation method of human epidermal melanocytes can be applied to investigate novel pathologies related to hyper- or hypo-pigmentary disorders.

Original language	English
Pages (from-to)	534-538
Number of pages	5
Journal	Pigment Cell and Melanoma Research
Volume	35
Issue number	5
DOIs	https://doi.org/10.1111/pcmr.13051
Publication status	Published - 2022 Sept

Bibliographical note

Funding Information:
This research was supported by the Brain Research Program through the National Research Foundation (NRF) funded by the Korean Ministry of Science, Information & Communications Technology (ICT) and Future Planning (NRF-2015M3C7A1028790) and Basic Science Research Program through the NRF funded by the Korean Ministry of Science and ICT (MSIT) (2020R1F1A1077244). Dr. Kennedy (Kennedy et al., 1999; Panoutsopoulou et al., 2009) kindly provided the original suction blister device, and the modified prototype product was pilot-produced by Aligned Genetics Inc., formerly named Logos biosystems Inc.

Funding Information:
This research was supported by the Brain Research Program through the National Research Foundation (NRF) funded by the Korean Ministry of Science, Information & Communications Technology (ICT) and Future Planning (NRF‐2015M3C7A1028790) and Basic Science Research Program through the NRF funded by the Korean Ministry of Science and ICT (MSIT) (2020R1F1A1077244). Dr. Kennedy (Kennedy et al., 1999 ; Panoutsopoulou et al., 2009 ) kindly provided the original suction blister device, and the modified prototype product was pilot‐produced by Aligned Genetics Inc., formerly named Logos biosystems Inc.

Publisher Copyright:
© 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Keywords

3-D imaging
melanocyte
nevus
skin-clearing technique
suction blister

ASJC Scopus subject areas

Oncology
Biochemistry, Genetics and Molecular Biology(all)
Dermatology

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10.1111/pcmr.13051

Cite this

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title = "Three-dimensional imaging for the analysis of human epidermal melanocytes",

abstract = "Three-dimensional (3-D) analysis of human epidermal melanocytes is required for deeper understanding of melanocytic disorders. The purpose of this study was to standardize 3-D imaging and quantification for the evaluation of epidermal melanocytes. The epidermal specimen was obtained using the suction blister method from a patient with melanocytic nevus on the forearm skin. Cutaneous ACT-PRESTO, the tissue-clearing and labeling technique, was subsequently performed. With the 3-D image analysis program, morphological reconstruction and quantification of selected perilesional and melanocytic nevus areas were possible. The region of melanocytic nevus showed higher numbers of total melanocytic dendrites and similar numbers of cell bodies compared with perilesional area. In addition, the mean area and volume of cell bodies increased in the melanocytic nevus area compared with the results in the perilesional area. The 3-D evaluation method of human epidermal melanocytes can be applied to investigate novel pathologies related to hyper- or hypo-pigmentary disorders.",

keywords = "3-D imaging, melanocyte, nevus, skin-clearing technique, suction blister",

author = "Kim, {Dai Hyun} and Lee, {Se Jeong} and Soo-Hong Seo and Ahn, {Hyo Hyun} and Kim, {Byung Jo} and Woong Sun and Rhyu, {Im Joo}",

note = "Funding Information: This research was supported by the Brain Research Program through the National Research Foundation (NRF) funded by the Korean Ministry of Science, Information & Communications Technology (ICT) and Future Planning (NRF-2015M3C7A1028790) and Basic Science Research Program through the NRF funded by the Korean Ministry of Science and ICT (MSIT) (2020R1F1A1077244). Dr. Kennedy (Kennedy et al., 1999; Panoutsopoulou et al., 2009) kindly provided the original suction blister device, and the modified prototype product was pilot-produced by Aligned Genetics Inc., formerly named Logos biosystems Inc. Funding Information: This research was supported by the Brain Research Program through the National Research Foundation (NRF) funded by the Korean Ministry of Science, Information & Communications Technology (ICT) and Future Planning (NRF‐2015M3C7A1028790) and Basic Science Research Program through the NRF funded by the Korean Ministry of Science and ICT (MSIT) (2020R1F1A1077244). Dr. Kennedy (Kennedy et al., 1999 ; Panoutsopoulou et al., 2009 ) kindly provided the original suction blister device, and the modified prototype product was pilot‐produced by Aligned Genetics Inc., formerly named Logos biosystems Inc. Publisher Copyright: {\textcopyright} 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.",

year = "2022",

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doi = "10.1111/pcmr.13051",

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AU - Kim, Byung Jo

AU - Sun, Woong

AU - Rhyu, Im Joo

N1 - Funding Information: This research was supported by the Brain Research Program through the National Research Foundation (NRF) funded by the Korean Ministry of Science, Information & Communications Technology (ICT) and Future Planning (NRF-2015M3C7A1028790) and Basic Science Research Program through the NRF funded by the Korean Ministry of Science and ICT (MSIT) (2020R1F1A1077244). Dr. Kennedy (Kennedy et al., 1999; Panoutsopoulou et al., 2009) kindly provided the original suction blister device, and the modified prototype product was pilot-produced by Aligned Genetics Inc., formerly named Logos biosystems Inc. Funding Information: This research was supported by the Brain Research Program through the National Research Foundation (NRF) funded by the Korean Ministry of Science, Information & Communications Technology (ICT) and Future Planning (NRF‐2015M3C7A1028790) and Basic Science Research Program through the NRF funded by the Korean Ministry of Science and ICT (MSIT) (2020R1F1A1077244). Dr. Kennedy (Kennedy et al., 1999 ; Panoutsopoulou et al., 2009 ) kindly provided the original suction blister device, and the modified prototype product was pilot‐produced by Aligned Genetics Inc., formerly named Logos biosystems Inc. Publisher Copyright: © 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

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N2 - Three-dimensional (3-D) analysis of human epidermal melanocytes is required for deeper understanding of melanocytic disorders. The purpose of this study was to standardize 3-D imaging and quantification for the evaluation of epidermal melanocytes. The epidermal specimen was obtained using the suction blister method from a patient with melanocytic nevus on the forearm skin. Cutaneous ACT-PRESTO, the tissue-clearing and labeling technique, was subsequently performed. With the 3-D image analysis program, morphological reconstruction and quantification of selected perilesional and melanocytic nevus areas were possible. The region of melanocytic nevus showed higher numbers of total melanocytic dendrites and similar numbers of cell bodies compared with perilesional area. In addition, the mean area and volume of cell bodies increased in the melanocytic nevus area compared with the results in the perilesional area. The 3-D evaluation method of human epidermal melanocytes can be applied to investigate novel pathologies related to hyper- or hypo-pigmentary disorders.

AB - Three-dimensional (3-D) analysis of human epidermal melanocytes is required for deeper understanding of melanocytic disorders. The purpose of this study was to standardize 3-D imaging and quantification for the evaluation of epidermal melanocytes. The epidermal specimen was obtained using the suction blister method from a patient with melanocytic nevus on the forearm skin. Cutaneous ACT-PRESTO, the tissue-clearing and labeling technique, was subsequently performed. With the 3-D image analysis program, morphological reconstruction and quantification of selected perilesional and melanocytic nevus areas were possible. The region of melanocytic nevus showed higher numbers of total melanocytic dendrites and similar numbers of cell bodies compared with perilesional area. In addition, the mean area and volume of cell bodies increased in the melanocytic nevus area compared with the results in the perilesional area. The 3-D evaluation method of human epidermal melanocytes can be applied to investigate novel pathologies related to hyper- or hypo-pigmentary disorders.

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Three-dimensional imaging for the analysis of human epidermal melanocytes

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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