Three-dimensional structure of the cytoskeleton in Trichomonas vaginalis revealed new features

Kyung Eun Lee; Ji Hui Kim; Min Kyo Jung; Tatsuo Arii; Jae Sook Ryu; Sung Sik Han

doi:10.1093/jmicro/dfp019

Three-dimensional structure of the cytoskeleton in Trichomonas vaginalis revealed new features

Kyung Eun Lee, Ji Hui Kim, Min Kyo Jung, Tatsuo Arii, Jae Sook Ryu, Sung Sik Han

* Honorary professors

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The flagellated protozoan Trichomonas vaginalis has been widely studied owing to its medical significance and unique structure. The complicated three-dimensional (3D) structure of the cellular components of T. vaginalis was reconstructed from serial sections to enable observation of the spatial features of the whole cell. Electron tomography was used to examine the detailed structure of the cellular organelles. Tomographic reconstruction showed the mastigont system and the parabasal filament of T. vaginalis in detail. The last thin filament (Pf3) was located close to the adjacent filament, and the two filaments appeared to be vertically parallel in the cross-sectional view. It is likely that Pf3 cannot be distinguished from the adjacent filament in 2D images obtained from transmission electron microscopy. Our 3D reconstruction of T. vaginalis revealed the presence of an additional striated fiber, and 3D reconstruction by electron tomography showed twisting of the split parabasal filament.

Original language	English
Pages (from-to)	305-313
Number of pages	9
Journal	Journal of Electron Microscopy
Volume	58
Issue number	5
DOIs	https://doi.org/10.1093/jmicro/dfp019
Publication status	Published - 2009 Oct

Keywords

3D structure
Additional parabasal filament
Electron tomography
Flagellar beating
T. vaginalis
Twisting structure

ASJC Scopus subject areas

Instrumentation

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10.1093/jmicro/dfp019

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@article{06c0a1c6b99c479683a22f2fe0c8a8f1,

title = "Three-dimensional structure of the cytoskeleton in Trichomonas vaginalis revealed new features",

abstract = "The flagellated protozoan Trichomonas vaginalis has been widely studied owing to its medical significance and unique structure. The complicated three-dimensional (3D) structure of the cellular components of T. vaginalis was reconstructed from serial sections to enable observation of the spatial features of the whole cell. Electron tomography was used to examine the detailed structure of the cellular organelles. Tomographic reconstruction showed the mastigont system and the parabasal filament of T. vaginalis in detail. The last thin filament (Pf3) was located close to the adjacent filament, and the two filaments appeared to be vertically parallel in the cross-sectional view. It is likely that Pf3 cannot be distinguished from the adjacent filament in 2D images obtained from transmission electron microscopy. Our 3D reconstruction of T. vaginalis revealed the presence of an additional striated fiber, and 3D reconstruction by electron tomography showed twisting of the split parabasal filament.",

keywords = "3D structure, Additional parabasal filament, Electron tomography, Flagellar beating, T. vaginalis, Twisting structure",

author = "Lee, {Kyung Eun} and Kim, {Ji Hui} and Jung, {Min Kyo} and Tatsuo Arii and Ryu, {Jae Sook} and Han, {Sung Sik}",

note = "Funding Information: This work was supported by the Korea Basic Sciences Institute (RKB-2005-000-00007-0-2006), MoST (R01-2005-000-10035-0) and Seoul Development Institute.",

year = "2009",

month = oct,

doi = "10.1093/jmicro/dfp019",

language = "English",

volume = "58",

pages = "305--313",

journal = "Journal of Electron Microscopy",

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publisher = "Japanese Society of Microscopy",

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AU - Lee, Kyung Eun

AU - Kim, Ji Hui

AU - Jung, Min Kyo

AU - Arii, Tatsuo

AU - Ryu, Jae Sook

AU - Han, Sung Sik

N1 - Funding Information: This work was supported by the Korea Basic Sciences Institute (RKB-2005-000-00007-0-2006), MoST (R01-2005-000-10035-0) and Seoul Development Institute.

PY - 2009/10

Y1 - 2009/10

N2 - The flagellated protozoan Trichomonas vaginalis has been widely studied owing to its medical significance and unique structure. The complicated three-dimensional (3D) structure of the cellular components of T. vaginalis was reconstructed from serial sections to enable observation of the spatial features of the whole cell. Electron tomography was used to examine the detailed structure of the cellular organelles. Tomographic reconstruction showed the mastigont system and the parabasal filament of T. vaginalis in detail. The last thin filament (Pf3) was located close to the adjacent filament, and the two filaments appeared to be vertically parallel in the cross-sectional view. It is likely that Pf3 cannot be distinguished from the adjacent filament in 2D images obtained from transmission electron microscopy. Our 3D reconstruction of T. vaginalis revealed the presence of an additional striated fiber, and 3D reconstruction by electron tomography showed twisting of the split parabasal filament.

AB - The flagellated protozoan Trichomonas vaginalis has been widely studied owing to its medical significance and unique structure. The complicated three-dimensional (3D) structure of the cellular components of T. vaginalis was reconstructed from serial sections to enable observation of the spatial features of the whole cell. Electron tomography was used to examine the detailed structure of the cellular organelles. Tomographic reconstruction showed the mastigont system and the parabasal filament of T. vaginalis in detail. The last thin filament (Pf3) was located close to the adjacent filament, and the two filaments appeared to be vertically parallel in the cross-sectional view. It is likely that Pf3 cannot be distinguished from the adjacent filament in 2D images obtained from transmission electron microscopy. Our 3D reconstruction of T. vaginalis revealed the presence of an additional striated fiber, and 3D reconstruction by electron tomography showed twisting of the split parabasal filament.

KW - 3D structure

KW - Additional parabasal filament

KW - Electron tomography

KW - Flagellar beating

KW - T. vaginalis

KW - Twisting structure

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U2 - 10.1093/jmicro/dfp019

DO - 10.1093/jmicro/dfp019

M3 - Article

C2 - 19386993

AN - SCOPUS:70349471184

SN - 0022-0744

VL - 58

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EP - 313

JO - Journal of Electron Microscopy

JF - Journal of Electron Microscopy

IS - 5

ER -

Three-dimensional structure of the cytoskeleton in Trichomonas vaginalis revealed new features

Abstract

Keywords

ASJC Scopus subject areas

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