Thymosin beta-4 promotes mesenchymal stem cell proliferation via an interleukin-8-dependent mechanism

Byung Joon Jeon, Yoolhee Yang, Su Kyung Shim, Heung Mo Yang, Daeho Cho, Sa Ik Bang

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSCs) hold great promise for the field of tissue regeneration. Because only a limited number of MSCs can be obtained from each donor site, it is important to establish standard methods for MSC expansion using growth and trophic factors. Thymosin β4 (Tβ4) is a novel trophic factor that has antimicrobial effects and the potential to promote tissue repair. Tβ4 is a ubiquitous, naturally-occurring peptide in the wound bed. Therefore, the relationship between Tβ4 and MSCs, especially adjacent adipose tissue-derived stem cells (ASCs), merits consideration. Exogenous Tβ4 treatment enhanced the proliferation of human ASCs, resulting in prominent nuclear localization of PCNA immunoreactivity. In addition, exogenous Tβ4 also increased IL-8 secretion and blocking of IL-8 with neutralizing antibodies decreased Tβ4-induced ASC proliferation, suggesting that IL-8 is a critical mediator of Tβ4-enhanced proliferation. Moreover, Tβ4 activated phosphorylation of ERK1/2 and increased the nuclear translocation of NF-κB. These observation provide that Tβ4 promotes the expansion of human ASCs via an IL-8-dependent mechanism that involves the ERK and NF-κB pathways. Therefore, Tβ4 could be used as a tool for MSC expansion in cell therapeutics.

Original languageEnglish
Pages (from-to)2526-2534
Number of pages9
JournalExperimental Cell Research
Volume319
Issue number17
DOIs
Publication statusPublished - 2013 Oct 15
Externally publishedYes

Bibliographical note

Funding Information:
This study was supported by a Samsung Biomedical Research Institute grant ( SBRI C-A9-214-3 ), by the Bio-Med Translational Research Center at Samsung Medical Center, and by the Korea Science and Engineering Foundation (KOSEF) through the SRC program (Research Center for Women's Disease) at Sookmyung Women's University.

Keywords

  • Cell proliferation
  • ERK pathway, NF-κB
  • Interleukin-8
  • Mesenchymal stem cells
  • Thymosin β4

ASJC Scopus subject areas

  • Cell Biology

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