Abstract
We previously demonstrated that TIMP-2 treatment of human microvascular endothelial cells (hMVECs) activates Rap1 via the pathway of paxillin-Crk-C3G. Here, we show that TIMP-2 overexpression in hMVECs by adenoviral infection enhances Rap1 expression, leading to further increase in Rap1-GTP. TIMP-2 expression, previously reported to inhibit cell migration, also leads to cell spreading accompanied with increased cell adhesion. HMVECs stably expressing Rap1 display a similar phenotype as hMVECs-TIMP-2, whereas the expression of inactive Rap1 mutant, Rap1(38N), leads to elongated appearance with greatly reduced cell adhesion. Furthermore, the phenotype of hMVECs-Rap1(38N) was not reversed by TIMP-2 overexpression. TIMP-2 greatly promotes the association of Rap1 with actin. Therefore, these findings suggest that TIMP-2 mediated alteration in cell morphology requires Rap1, TIMP-2 may recruit Rap1 to sites of actin cytoskeleton remodeling necessary for cell spreading, and enhanced cell adhesion by TIMP-2 expression may hinder cell migration.
Original language | English |
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Pages (from-to) | 1201-1206 |
Number of pages | 6 |
Journal | Biochemical and biophysical research communications |
Volume | 345 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2006 Jul 7 |
Bibliographical note
Funding Information:This work was supported by intramural research funds from the National Cancer Institute, Center for Cancer Research (Project # Z01SC 009179) and by grant from BAERI (M2-0508-02-0000-05-B08-02-000-00) of National Nuclear R&D Program from Ministry of Science and Technology of Korea.
Keywords
- Cell adhesion
- Cell migration
- Cell spreading
- Rap1
- TIMP-2
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology