Tolerance of Saccharomyces cerevisiae K35 to lignocellulose-derived inhibitory compounds

Hawon Lee, Dae Haeng Cho, Yong Hwan Kim, Soo Jeong Shin, Sung Bong Kim, Sung Ok Han, Jinwon Lee, Seung Wook Kim, Chulhwan Park

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)


The hydrolysis which converts polysaccharides to the fermentable sugars for yeast's lingocellulosic ethanol production also generates byproducts which inhibit the ethanol production. To investigate the extent to which inhibitory compounds affect yeast's growth and ethanol production, fermentations by Saccharomyces cerevisiae K35 were investigated in various concentrations of acetic acid, furfural, 5-hydroxymethylfurfural (5-HMF), syringaldehyde, and coumaric acid. Fermentation in hydrolysates from yellow poplar and waste wood was also studied. After 24 h, S. cerevisiae K35 produced close to theoretically predicted ethanol yields in all the concentrations of acetic acid tested (1 ∼ 10 g/L). Both furans and phenolics inhibited cell growth and ethanol production. Ethanol yield, however, was unaffected, even at high concentrations, except in the cases of 5 g/L of syringaldehyde and coumaric acid. Although hydrolysates contain various toxic compounds, in their presence, S. Cerevisiae K35 consumed close to all the available glucose and yielded more ethanol than theoretically predicted. S. Cerevisiae K35 was demonstrated to have high tolerance to inhibitory compounds and not to need any detoxification for ethanol production from hydrolysates.

Original languageEnglish
Pages (from-to)755-760
Number of pages6
JournalBiotechnology and Bioprocess Engineering
Issue number4
Publication statusPublished - 2011 Aug

Bibliographical note

Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0002313) and by the Research Grant from Kwangwoon University in 2011. This research was also supported by the Technology Development Program (309016-5) for Agriculture and Forestry of the Ministry for Food, Agriculture, Forestry and Fisheries of Korea.


  • Saccharomyces cerevisiae
  • ethanol
  • hydrolysate
  • inhibitor
  • lignocelluloses

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering


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