Tomographic phase microscopy

Wonshik Choi, Christopher Fang-Yen, Kamran Badizadegan, Seungeun Oh, Niyom Lue, Ramachandra R. Dasari, Michael S. Feld

Research output: Contribution to journalArticlepeer-review

922 Citations (Scopus)

Abstract

We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.

Original languageEnglish
Pages (from-to)717-719
Number of pages3
JournalNature Methods
Volume4
Issue number9
DOIs
Publication statusPublished - 2007 Sept

Bibliographical note

Funding Information:
This work was funded by US National Institutes of Health (P41-RR02594-18) and Hamamatsu Corporation.

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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