Abstract
We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.
Original language | English |
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Pages (from-to) | 717-719 |
Number of pages | 3 |
Journal | Nature Methods |
Volume | 4 |
Issue number | 9 |
DOIs | |
Publication status | Published - 2007 Sept |
Bibliographical note
Funding Information:This work was funded by US National Institutes of Health (P41-RR02594-18) and Hamamatsu Corporation.
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Molecular Biology
- Cell Biology