Tomographic phase microscopy and its biological applications

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13 Citations (Scopus)


Conventional interferometric microscopy techniques such as digital holographic microscopy and quantitative phase microscopy are often classified as 3D imaging techniques because a recorded complex field image can be numerically propagated to a different depth. In a strict sense, however, a single complex field image contains only 2D information on a specimen. The measured 2D image is only a subset of the 3D structure. For the 3D mapping of an object, multiple independent 2D images are to be taken, for example at multiple incident angles or wavelengths, and then combined by the so-called optical diffraction tomography (ODT). In this Letter, tomographic phase microscopy (TPM) is reviewed that experimentally realizes the concept of the ODT for the 3D mapping of biological cells in their native state, and some of its interesting biological and biomedical applications are introduced.[Figure not available: see fulltext.]

Original languageEnglish
Article number5
Pages (from-to)1-11
Number of pages11
Journal3D Research
Issue number4
Publication statusPublished - 2012


  • 3D imaging
  • Optical diffraction tomography
  • digital holographic microscopy
  • label-free imaging

ASJC Scopus subject areas

  • Software
  • Electrical and Electronic Engineering


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