Tomographic phase microscopy

Wonshik Choi; Christopher Fang-Yen; Kamran Badizadegan; Seungeun Oh; Niyom Lue; Ramachandra R. Dasari; Michael S. Feld

doi:10.1038/nmeth1078

Tomographic phase microscopy

Wonshik Choi
, Christopher Fang-Yen
, Kamran Badizadegan
, Seungeun Oh
, Niyom Lue
, Ramachandra R. Dasari
, Michael S. Feld^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

970 Citations (Scopus)

Abstract

We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.

Original language	English
Pages (from-to)	717-719
Number of pages	3
Journal	Nature Methods
Volume	4
Issue number	9
DOIs	https://doi.org/10.1038/nmeth1078
Publication status	Published - 2007 Sept

Bibliographical note

Funding Information:
This work was funded by US National Institutes of Health (P41-RR02594-18) and Hamamatsu Corporation.

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth1078

Cite this

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title = "Tomographic phase microscopy",

abstract = "We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.",

author = "Wonshik Choi and Christopher Fang-Yen and Kamran Badizadegan and Seungeun Oh and Niyom Lue and Dasari, \{Ramachandra R.\} and Feld, \{Michael S.\}",

note = "Funding Information: This work was funded by US National Institutes of Health (P41-RR02594-18) and Hamamatsu Corporation.",

year = "2007",

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doi = "10.1038/nmeth1078",

language = "English",

volume = "4",

pages = "717--719",

journal = "Nature Methods",

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T1 - Tomographic phase microscopy

AU - Choi, Wonshik

AU - Fang-Yen, Christopher

AU - Badizadegan, Kamran

AU - Oh, Seungeun

AU - Lue, Niyom

AU - Dasari, Ramachandra R.

AU - Feld, Michael S.

N1 - Funding Information: This work was funded by US National Institutes of Health (P41-RR02594-18) and Hamamatsu Corporation.

PY - 2007/9

Y1 - 2007/9

N2 - We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.

AB - We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.

UR - https://www.scopus.com/pages/publications/34548316112

U2 - 10.1038/nmeth1078

DO - 10.1038/nmeth1078

M3 - Article

C2 - 17694065

AN - SCOPUS:34548316112

SN - 1548-7091

VL - 4

SP - 717

EP - 719

JO - Nature Methods

JF - Nature Methods

IS - 9

ER -

Tomographic phase microscopy

Abstract

Bibliographical note

ASJC Scopus subject areas

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