TOPLESS mediates brassinosteroid-induced transcriptional repression through interaction with BZR1

Eunkyoo Oh; Jia Ying Zhu; Hojin Ryu; Ildoo Hwang; Zhi Yong Wang

doi:10.1038/ncomms5140

TOPLESS mediates brassinosteroid-induced transcriptional repression through interaction with BZR1

Eunkyoo Oh, Jia Ying Zhu, Hojin Ryu, Ildoo Hwang, Zhi Yong Wang

Research output: Contribution to journal › Article › peer-review

113 Citations (Scopus)

Abstract

Brassinosteroid (BR) regulates plant development by activating the transcription factor brassinazole resistant 1 (BZR1), which activates and represses different target genes to switch cellular programmes. The mechanisms that determine BZR1â ™ s transcriptional activities remain largely unknown. Here we show that BZR1 represses target genes by recruiting the Groucho/TUP1-like transcriptional corepressor TOPLESS (TPL). Specific deletion or mutation of an evolutionarily conserved ERF-associated amphiphilic repression (EAR) motif at the carboxy terminus abolishes BZR1â ™ s abilities to regulate gene expression and cell elongation, but these defects are rescued by TPL fusion to the EAR motif-mutated BZR1. The EAR motif in BZR1 mediates recruitment of TPL to BZR1-repressed promoters. A triple tpl mutant (tpl;tpr1;tpr4) shows reduced BR sensitivity and suppresses the gain-of-function bzr1-1D mutant phenotype. BR repression of gene expression also requires histone deacetylases that interact with TPL. Our study demonstrates key roles of the EAR motif and TPL in BR regulation of gene expression and plant growth.

Original language	English
Article number	4140
Journal	Nature communications
Volume	5
DOIs	https://doi.org/10.1038/ncomms5140
Publication status	Published - 2014 Jun 18
Externally published	Yes

Bibliographical note

Funding Information:
We thank Srinivas S. Gampala and Josh M. Gendron for generating the bzs285 mutant, Yuelin Zhang for providing seeds of the tpl;tpr1;tpr4 triple mutant and David E. Somers for providing TPLp::TPL-HA seeds. The research was supported by a grant from NIH (R01GM066258) to Z.-Y.W. H.R. was supported by a grant from the National Research Foundation (2013R1A1A1076010) funded by the Korean Ministry of Science, ICT and Future Planning. I.H. was supported by the Next Generation Biogreen 21 programme (PJ009516).

ASJC Scopus subject areas

General Chemistry
General Biochemistry,Genetics and Molecular Biology
General Physics and Astronomy

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title = "TOPLESS mediates brassinosteroid-induced transcriptional repression through interaction with BZR1",

abstract = "Brassinosteroid (BR) regulates plant development by activating the transcription factor brassinazole resistant 1 (BZR1), which activates and represses different target genes to switch cellular programmes. The mechanisms that determine BZR1{\^a} {\texttrademark} s transcriptional activities remain largely unknown. Here we show that BZR1 represses target genes by recruiting the Groucho/TUP1-like transcriptional corepressor TOPLESS (TPL). Specific deletion or mutation of an evolutionarily conserved ERF-associated amphiphilic repression (EAR) motif at the carboxy terminus abolishes BZR1{\^a} {\texttrademark} s abilities to regulate gene expression and cell elongation, but these defects are rescued by TPL fusion to the EAR motif-mutated BZR1. The EAR motif in BZR1 mediates recruitment of TPL to BZR1-repressed promoters. A triple tpl mutant (tpl;tpr1;tpr4) shows reduced BR sensitivity and suppresses the gain-of-function bzr1-1D mutant phenotype. BR repression of gene expression also requires histone deacetylases that interact with TPL. Our study demonstrates key roles of the EAR motif and TPL in BR regulation of gene expression and plant growth.",

author = "Eunkyoo Oh and Zhu, {Jia Ying} and Hojin Ryu and Ildoo Hwang and Wang, {Zhi Yong}",

note = "Funding Information: We thank Srinivas S. Gampala and Josh M. Gendron for generating the bzs285 mutant, Yuelin Zhang for providing seeds of the tpl;tpr1;tpr4 triple mutant and David E. Somers for providing TPLp::TPL-HA seeds. The research was supported by a grant from NIH (R01GM066258) to Z.-Y.W. H.R. was supported by a grant from the National Research Foundation (2013R1A1A1076010) funded by the Korean Ministry of Science, ICT and Future Planning. I.H. was supported by the Next Generation Biogreen 21 programme (PJ009516).",

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AU - Wang, Zhi Yong

N1 - Funding Information: We thank Srinivas S. Gampala and Josh M. Gendron for generating the bzs285 mutant, Yuelin Zhang for providing seeds of the tpl;tpr1;tpr4 triple mutant and David E. Somers for providing TPLp::TPL-HA seeds. The research was supported by a grant from NIH (R01GM066258) to Z.-Y.W. H.R. was supported by a grant from the National Research Foundation (2013R1A1A1076010) funded by the Korean Ministry of Science, ICT and Future Planning. I.H. was supported by the Next Generation Biogreen 21 programme (PJ009516).

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N2 - Brassinosteroid (BR) regulates plant development by activating the transcription factor brassinazole resistant 1 (BZR1), which activates and represses different target genes to switch cellular programmes. The mechanisms that determine BZR1â ™ s transcriptional activities remain largely unknown. Here we show that BZR1 represses target genes by recruiting the Groucho/TUP1-like transcriptional corepressor TOPLESS (TPL). Specific deletion or mutation of an evolutionarily conserved ERF-associated amphiphilic repression (EAR) motif at the carboxy terminus abolishes BZR1â ™ s abilities to regulate gene expression and cell elongation, but these defects are rescued by TPL fusion to the EAR motif-mutated BZR1. The EAR motif in BZR1 mediates recruitment of TPL to BZR1-repressed promoters. A triple tpl mutant (tpl;tpr1;tpr4) shows reduced BR sensitivity and suppresses the gain-of-function bzr1-1D mutant phenotype. BR repression of gene expression also requires histone deacetylases that interact with TPL. Our study demonstrates key roles of the EAR motif and TPL in BR regulation of gene expression and plant growth.

AB - Brassinosteroid (BR) regulates plant development by activating the transcription factor brassinazole resistant 1 (BZR1), which activates and represses different target genes to switch cellular programmes. The mechanisms that determine BZR1â ™ s transcriptional activities remain largely unknown. Here we show that BZR1 represses target genes by recruiting the Groucho/TUP1-like transcriptional corepressor TOPLESS (TPL). Specific deletion or mutation of an evolutionarily conserved ERF-associated amphiphilic repression (EAR) motif at the carboxy terminus abolishes BZR1â ™ s abilities to regulate gene expression and cell elongation, but these defects are rescued by TPL fusion to the EAR motif-mutated BZR1. The EAR motif in BZR1 mediates recruitment of TPL to BZR1-repressed promoters. A triple tpl mutant (tpl;tpr1;tpr4) shows reduced BR sensitivity and suppresses the gain-of-function bzr1-1D mutant phenotype. BR repression of gene expression also requires histone deacetylases that interact with TPL. Our study demonstrates key roles of the EAR motif and TPL in BR regulation of gene expression and plant growth.

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TOPLESS mediates brassinosteroid-induced transcriptional repression through interaction with BZR1

Abstract

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