Topographical guidance of mouse neuronal cell on SiO2 microtracks

Jin Woo Lee, Kyong Soo Lee, Nana Cho, Byeong Kwon Ju, Kyu Back Lee, Sang Ho Lee

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)


Three different microtrack patterns were developed on SiO2 layer deposited on a quartz wafer for the observation of topographical contact guidance of living neurons. The patterns were linear, dashed, and zigzag types. Mouse N2a neuroblastoma cells were cultured on the pattern and differentiated into neuronal cells after 4 days in the presence of dibutyryl cyclic AMP (dbcAMP) showing several neurites and growth cones. Among the three patterns, zigzag type pattern was the most effective for directional guiding of neuonal growth and motility. Two different types of growth cones - growth cone with relatively long filopodia and with large lamellipodia - were observed at the ends of axon shafts using a scanning electron microscope. Our research showed that continuous and complex pattern is more favorable for the stimulation of neuronal cell growth and motility, which will be helpful for the design and development of artificial tissues.

Original languageEnglish
Pages (from-to)252-257
Number of pages6
JournalSensors and Actuators, B: Chemical
Issue number1
Publication statusPublished - 2007 Dec 12

Bibliographical note

Funding Information:
This work was supported in part by the Brain Korea 21 Project in 2007, by the Seoul R&BD Program, and by the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (Grant A050750 and Grant 02-PJ3-PG6-EV09-0001).


  • Axon shaft
  • Growth cone
  • Microtrack
  • N2a neuroblastoma
  • Topographical guidance

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry


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