Toxic effects of titanium dioxide nanoparticles on microbial activity and metabolic flux

Jinwon Lee; Soohyun Park; Soojin Lee; Borim Kim; Sunhee Lee; Juhee Lei; Sangjun Sim; Manbock Gu; Jongheop Yi

doi:10.1007/s12257-010-0251-4

Toxic effects of titanium dioxide nanoparticles on microbial activity and metabolic flux

Jinwon Lee, Soohyun Park, Soojin Lee, Borim Kim, Sunhee Lee, Juhee Lei, Sangjun Sim, Manbock Gu, Jongheop Yi

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

The purpose of this research is to estimate and quantify the toxicity of titanium dioxide (TiO₂) nanoparticles in microorganisms. Nano-sized particles of TiO₂ were more toxic compared to micro-sized particles. Three microorganismal species, Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae, were used to test TiO₂ antimicrobial effects. E. coli showed the lowest survival rate (36%), while S. cerevisiae showed the highest survival rate (71%). The antimicrobial effect of TiO ₂ was also dependent on ultraviolet ray wavelength. The survival ratio of E. coli was 40% at a 254 nm wavelength and 80% at 365 nm. To observe the effect of TiO2 on the intracellular metabolism, a metabolic flux analysis and the measurement of in vivo glucose-6-phosphate were performed. G6P concentration in cells exposed to TiO₂ increased, and glycolysis flux was also higher than the controls.

Original language	English
Pages (from-to)	276-282
Number of pages	7
Journal	Biotechnology and Bioprocess Engineering
Volume	17
Issue number	2
DOIs	https://doi.org/10.1007/s12257-010-0251-4
Publication status	Published - 2012 Apr

Bibliographical note

Funding Information:
This work was supported by the Ministry of Environment as “The Eco-technopia 21 project” and by the Second Stage of Brain Korea 21 Project (Ministry of Education & Human Resources Development). This research was supported by the R&D Program of MKE/KEIT (No. 10035578, Development of 2,3-butanediol and derivative production technology for C-Zero bio-platform industry). This work was supported by the Graduate School of Specialization for Biotechnology Program of the Ministry of Knowledge Economy (MKE).

Keywords

Antimicrobial activity
Glucose-6-phosphate
Metabolic flux analysis
TiO2 nanoparticles

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Biomedical Engineering

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10.1007/s12257-010-0251-4

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title = "Toxic effects of titanium dioxide nanoparticles on microbial activity and metabolic flux",

abstract = "The purpose of this research is to estimate and quantify the toxicity of titanium dioxide (TiO2) nanoparticles in microorganisms. Nano-sized particles of TiO2 were more toxic compared to micro-sized particles. Three microorganismal species, Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae, were used to test TiO2 antimicrobial effects. E. coli showed the lowest survival rate (36%), while S. cerevisiae showed the highest survival rate (71%). The antimicrobial effect of TiO 2 was also dependent on ultraviolet ray wavelength. The survival ratio of E. coli was 40% at a 254 nm wavelength and 80% at 365 nm. To observe the effect of TiO2 on the intracellular metabolism, a metabolic flux analysis and the measurement of in vivo glucose-6-phosphate were performed. G6P concentration in cells exposed to TiO2 increased, and glycolysis flux was also higher than the controls.",

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author = "Jinwon Lee and Soohyun Park and Soojin Lee and Borim Kim and Sunhee Lee and Juhee Lei and Sangjun Sim and Manbock Gu and Jongheop Yi",

note = "Funding Information: This work was supported by the Ministry of Environment as “The Eco-technopia 21 project” and by the Second Stage of Brain Korea 21 Project (Ministry of Education & Human Resources Development). This research was supported by the R&D Program of MKE/KEIT (No. 10035578, Development of 2,3-butanediol and derivative production technology for C-Zero bio-platform industry). This work was supported by the Graduate School of Specialization for Biotechnology Program of the Ministry of Knowledge Economy (MKE).",

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doi = "10.1007/s12257-010-0251-4",

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AU - Lee, Jinwon

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AU - Kim, Borim

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AU - Lei, Juhee

AU - Sim, Sangjun

AU - Gu, Manbock

AU - Yi, Jongheop

N1 - Funding Information: This work was supported by the Ministry of Environment as “The Eco-technopia 21 project” and by the Second Stage of Brain Korea 21 Project (Ministry of Education & Human Resources Development). This research was supported by the R&D Program of MKE/KEIT (No. 10035578, Development of 2,3-butanediol and derivative production technology for C-Zero bio-platform industry). This work was supported by the Graduate School of Specialization for Biotechnology Program of the Ministry of Knowledge Economy (MKE).

PY - 2012/4

Y1 - 2012/4

N2 - The purpose of this research is to estimate and quantify the toxicity of titanium dioxide (TiO2) nanoparticles in microorganisms. Nano-sized particles of TiO2 were more toxic compared to micro-sized particles. Three microorganismal species, Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae, were used to test TiO2 antimicrobial effects. E. coli showed the lowest survival rate (36%), while S. cerevisiae showed the highest survival rate (71%). The antimicrobial effect of TiO 2 was also dependent on ultraviolet ray wavelength. The survival ratio of E. coli was 40% at a 254 nm wavelength and 80% at 365 nm. To observe the effect of TiO2 on the intracellular metabolism, a metabolic flux analysis and the measurement of in vivo glucose-6-phosphate were performed. G6P concentration in cells exposed to TiO2 increased, and glycolysis flux was also higher than the controls.

AB - The purpose of this research is to estimate and quantify the toxicity of titanium dioxide (TiO2) nanoparticles in microorganisms. Nano-sized particles of TiO2 were more toxic compared to micro-sized particles. Three microorganismal species, Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae, were used to test TiO2 antimicrobial effects. E. coli showed the lowest survival rate (36%), while S. cerevisiae showed the highest survival rate (71%). The antimicrobial effect of TiO 2 was also dependent on ultraviolet ray wavelength. The survival ratio of E. coli was 40% at a 254 nm wavelength and 80% at 365 nm. To observe the effect of TiO2 on the intracellular metabolism, a metabolic flux analysis and the measurement of in vivo glucose-6-phosphate were performed. G6P concentration in cells exposed to TiO2 increased, and glycolysis flux was also higher than the controls.

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Toxic effects of titanium dioxide nanoparticles on microbial activity and metabolic flux

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Bibliographical note

Keywords

ASJC Scopus subject areas

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