Toxic effects of titanium dioxide nanoparticles on microbial activity and metabolic flux

Jinwon Lee; Soohyun Park; Soojin Lee; Borim Kim; Sunhee Lee; Juhee Lei; Sangjun Sim; Manbock Gu; Jongheop Yi

doi:10.1007/s12257-010-0251-4

Toxic effects of titanium dioxide nanoparticles on microbial activity and metabolic flux

Jinwon Lee^*, Soohyun Park, Soojin Lee, Borim Kim, Sunhee Lee, Juhee Lei, Sangjun Sim, Manbock Gu, Jongheop Yi

^*Corresponding author for this work

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

The purpose of this research is to estimate and quantify the toxicity of titanium dioxide (TiO₂) nanoparticles in microorganisms. Nano-sized particles of TiO₂ were more toxic compared to micro-sized particles. Three microorganismal species, Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae, were used to test TiO₂ antimicrobial effects. E. coli showed the lowest survival rate (36%), while S. cerevisiae showed the highest survival rate (71%). The antimicrobial effect of TiO ₂ was also dependent on ultraviolet ray wavelength. The survival ratio of E. coli was 40% at a 254 nm wavelength and 80% at 365 nm. To observe the effect of TiO2 on the intracellular metabolism, a metabolic flux analysis and the measurement of in vivo glucose-6-phosphate were performed. G6P concentration in cells exposed to TiO₂ increased, and glycolysis flux was also higher than the controls.

Original language	English
Pages (from-to)	276-282
Number of pages	7
Journal	Biotechnology and Bioprocess Engineering
Volume	17
Issue number	2
DOIs	https://doi.org/10.1007/s12257-010-0251-4
Publication status	Published - 2012 Apr

Bibliographical note

Funding Information:
This work was supported by the Ministry of Environment as “The Eco-technopia 21 project” and by the Second Stage of Brain Korea 21 Project (Ministry of Education & Human Resources Development). This research was supported by the R&D Program of MKE/KEIT (No. 10035578, Development of 2,3-butanediol and derivative production technology for C-Zero bio-platform industry). This work was supported by the Graduate School of Specialization for Biotechnology Program of the Ministry of Knowledge Economy (MKE).

Keywords

Antimicrobial activity
Glucose-6-phosphate
Metabolic flux analysis
TiO2 nanoparticles

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Biomedical Engineering

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10.1007/s12257-010-0251-4

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title = "Toxic effects of titanium dioxide nanoparticles on microbial activity and metabolic flux",

abstract = "The purpose of this research is to estimate and quantify the toxicity of titanium dioxide (TiO2) nanoparticles in microorganisms. Nano-sized particles of TiO2 were more toxic compared to micro-sized particles. Three microorganismal species, Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae, were used to test TiO2 antimicrobial effects. E. coli showed the lowest survival rate (36\%), while S. cerevisiae showed the highest survival rate (71\%). The antimicrobial effect of TiO 2 was also dependent on ultraviolet ray wavelength. The survival ratio of E. coli was 40\% at a 254 nm wavelength and 80\% at 365 nm. To observe the effect of TiO2 on the intracellular metabolism, a metabolic flux analysis and the measurement of in vivo glucose-6-phosphate were performed. G6P concentration in cells exposed to TiO2 increased, and glycolysis flux was also higher than the controls.",

keywords = "Antimicrobial activity, Glucose-6-phosphate, Metabolic flux analysis, TiO2 nanoparticles",

author = "Jinwon Lee and Soohyun Park and Soojin Lee and Borim Kim and Sunhee Lee and Juhee Lei and Sangjun Sim and Manbock Gu and Jongheop Yi",

note = "Funding Information: This work was supported by the Ministry of Environment as “The Eco-technopia 21 project” and by the Second Stage of Brain Korea 21 Project (Ministry of Education \& Human Resources Development). This research was supported by the R\&D Program of MKE/KEIT (No. 10035578, Development of 2,3-butanediol and derivative production technology for C-Zero bio-platform industry). This work was supported by the Graduate School of Specialization for Biotechnology Program of the Ministry of Knowledge Economy (MKE).",

year = "2012",

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doi = "10.1007/s12257-010-0251-4",

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AU - Lee, Jinwon

AU - Park, Soohyun

AU - Lee, Soojin

AU - Kim, Borim

AU - Lee, Sunhee

AU - Lei, Juhee

AU - Sim, Sangjun

AU - Gu, Manbock

AU - Yi, Jongheop

N1 - Funding Information: This work was supported by the Ministry of Environment as “The Eco-technopia 21 project” and by the Second Stage of Brain Korea 21 Project (Ministry of Education & Human Resources Development). This research was supported by the R&D Program of MKE/KEIT (No. 10035578, Development of 2,3-butanediol and derivative production technology for C-Zero bio-platform industry). This work was supported by the Graduate School of Specialization for Biotechnology Program of the Ministry of Knowledge Economy (MKE).

PY - 2012/4

Y1 - 2012/4

N2 - The purpose of this research is to estimate and quantify the toxicity of titanium dioxide (TiO2) nanoparticles in microorganisms. Nano-sized particles of TiO2 were more toxic compared to micro-sized particles. Three microorganismal species, Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae, were used to test TiO2 antimicrobial effects. E. coli showed the lowest survival rate (36%), while S. cerevisiae showed the highest survival rate (71%). The antimicrobial effect of TiO 2 was also dependent on ultraviolet ray wavelength. The survival ratio of E. coli was 40% at a 254 nm wavelength and 80% at 365 nm. To observe the effect of TiO2 on the intracellular metabolism, a metabolic flux analysis and the measurement of in vivo glucose-6-phosphate were performed. G6P concentration in cells exposed to TiO2 increased, and glycolysis flux was also higher than the controls.

AB - The purpose of this research is to estimate and quantify the toxicity of titanium dioxide (TiO2) nanoparticles in microorganisms. Nano-sized particles of TiO2 were more toxic compared to micro-sized particles. Three microorganismal species, Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae, were used to test TiO2 antimicrobial effects. E. coli showed the lowest survival rate (36%), while S. cerevisiae showed the highest survival rate (71%). The antimicrobial effect of TiO 2 was also dependent on ultraviolet ray wavelength. The survival ratio of E. coli was 40% at a 254 nm wavelength and 80% at 365 nm. To observe the effect of TiO2 on the intracellular metabolism, a metabolic flux analysis and the measurement of in vivo glucose-6-phosphate were performed. G6P concentration in cells exposed to TiO2 increased, and glycolysis flux was also higher than the controls.

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KW - TiO2 nanoparticles

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IS - 2

ER -

Toxic effects of titanium dioxide nanoparticles on microbial activity and metabolic flux

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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