Toxicity biomonitoring of degradation byproducts using freeze-dried recombinant bioluminescent bacteria

Sue Hyung Choi, Man Bock Gu

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37 Citations (Scopus)


A toxicity biomonitoring system using freeze-dried recombinant bioluminescent bacteria was implemented to diagnose the biotreatment of 2,4,5-trichlorophenol and its degradation byproducts of using a cell-free culture broth of Phanerochaete chrysosporium. The cellular toxicity was measured by the bioluminescence of constitutive bioluminescent bacteria, such as Photobacterium phosphoreum and GC2, while information on the toxicity caused by unknown byproducts was obtained using the specific bioluminescent responses of stress-inducible bioluminescent bacteria, which included DPD2540 (membrane-damage sensitive), TV1061 (protein-damage sensitive), DPD2794 (DNA-damage sensitive), and DPD2511 (oxidative-damage sensitive) strains. An overall decrease in the cellular toxicity was observed as the treatment progressed, i.e. as 2,4,5-trichlorophenol disappeared. On the other hand, bioluminescent responses from DPD2511, DPD2540, and TV1061 increased as degradation progressed, most probably due to the formation of byproducts causing oxidative-, membrane-, and protein-damage. In conclusion, this toxicity biomonitoring method may be applied to evaluate the toxicities of degradation byproducts of the other environmental processes to provide more information about the mode of the byproducts' toxicity.

Original languageEnglish
Pages (from-to)229-238
Number of pages10
JournalAnalytica Chimica Acta
Issue number2
Publication statusPublished - 2003 Apr 3
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by the National Research Laboratory (2001 NRL) Program of Korea Institute of Science and Technology Evaluation and Planning (Project No. M10104000094-01J000004100).

Copyright 2017 Elsevier B.V., All rights reserved.


  • Byproducts' toxicity
  • Diagnostic of biotreatment results
  • Freeze-dried bioluminescent bacteria
  • Toxicity classification
  • Toxicity monitoring

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy


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