Abstract
Five different freeze-dried recombinant bioluminescent bacteria were used for the detection of cellular stresses caused by endocrine disrupting chemicals. These strains were DPD2794 (recA::luxCDABE), which is sensitive to DNA damage, DPD2540 (fabA:: luxCDABE), sensitive to cellular membrane damage, DPD2511 (katG::luxCDABE), sensitive to oxidative damage, and TV1061 (grpE::luxCDABE), sensitive to protein damage. GC2, which emits bioluminescence constitutively, was also used in this study. The toxicity of several chemicals was determined on the first four freeze-dried bacteria, while nonspecific cellular stresses were measured using GC2. Damage caused by known endocrine disrupting chemicals, such as nonyl phenol, bisphenol A, and styrene, was detected and classified according to toxicity mode, while others, such as phathalate and DDT, were not detected with the bacteria. These results suggest that endocrine disrupting chemicals are toxic in bacteria, and do not act via an estrogenic effect, and that toxicity monitoring and classification of some endocrine dis-rupting chemicals may be possible in the field using these freeze-dried recombinant bioluminescent bacteria.
Original language | English |
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Pages (from-to) | 395-399 |
Number of pages | 5 |
Journal | Biotechnology and Bioprocess Engineering |
Volume | 5 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2000 |
Externally published | Yes |
Bibliographical note
Funding Information:Acknowledgements This work was supported by the Minister of the Environment of Korea through the G7 project, and in part by KOSEF through the Advanced Environmental Monitoring Research Center (ADEMRC) at the Kwangju Institute of Science and Technology (K-JIST). Authors are grateful for their support.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
Keywords
- Cellular toxicity
- Endocrine disrupting chemicals (EDCs)
- Freeze-dried recombinant bioluminescent bacteria
- Toxic effect
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
- Biomedical Engineering