Tracking and protection of transplanted stem cells using a ferrocenecarboxylic acid-conjugated peptide that mimics hTERT

Hyun Hee Park; Kyu Yong Lee; Dong Woo Park; Na Young Choi; Young Joo Lee; Jeong Woo Son; Sangjae Kim; Chanil Moon; Hyun Wook Kim; Im Joo Rhyu; Seong Ho Koh

doi:10.1016/j.biomaterials.2017.11.009

Tracking and protection of transplanted stem cells using a ferrocenecarboxylic acid-conjugated peptide that mimics hTERT

Hyun Hee Park, Kyu Yong Lee, Dong Woo Park, Na Young Choi, Young Joo Lee, Jeong Woo Son, Sangjae Kim, Chanil Moon, Hyun Wook Kim, Im Joo Rhyu, Seong Ho Koh

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

In vivo tracking of transplanted stem cells has been a central aim of stem cell therapy. Although many tracking systems have been introduced, no method has yet been validated for clinical applications. We developed a novel sophisticated peptide (GV1001) that mimics hTERT (human telomerase reverse transcriptase) and analysed its ability to track and protect stem cells after transplantation. Ferrocenecarboxylic acid-conjugated GV1001 (Fe-GV1001) efficiently penetrated stem cells with no adverse effects. Moreover, Fe-GV1001 improved the viability, proliferation, and migration of stem cells under hypoxia. After Fe-GV1001-labelled stem cells were transplanted into the brains of rats after stroke, the labelled cells were easily tracked by MRI. Our findings indicate that Fe-GV1001 can be used for the in vivo tracking of stem cells after transplantation into the brain and can improve the efficacy of stem cell therapy by sustaining and enhancing stem cell characteristics under disease conditions.

Original language	English
Pages (from-to)	80-91
Number of pages	12
Journal	Biomaterials
Volume	155
DOIs	https://doi.org/10.1016/j.biomaterials.2017.11.009
Publication status	Published - 2018 Feb

Keywords

MRI
Mesenchymal stem cells
Neural stem cells
Peptide
Tracking
hTERT

ASJC Scopus subject areas

Biophysics
Bioengineering
Ceramics and Composites
Biomaterials
Mechanics of Materials

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biomaterials.2017.11.009

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title = "Tracking and protection of transplanted stem cells using a ferrocenecarboxylic acid-conjugated peptide that mimics hTERT",

abstract = "In vivo tracking of transplanted stem cells has been a central aim of stem cell therapy. Although many tracking systems have been introduced, no method has yet been validated for clinical applications. We developed a novel sophisticated peptide (GV1001) that mimics hTERT (human telomerase reverse transcriptase) and analysed its ability to track and protect stem cells after transplantation. Ferrocenecarboxylic acid-conjugated GV1001 (Fe-GV1001) efficiently penetrated stem cells with no adverse effects. Moreover, Fe-GV1001 improved the viability, proliferation, and migration of stem cells under hypoxia. After Fe-GV1001-labelled stem cells were transplanted into the brains of rats after stroke, the labelled cells were easily tracked by MRI. Our findings indicate that Fe-GV1001 can be used for the in vivo tracking of stem cells after transplantation into the brain and can improve the efficacy of stem cell therapy by sustaining and enhancing stem cell characteristics under disease conditions.",

keywords = "MRI, Mesenchymal stem cells, Neural stem cells, Peptide, Tracking, hTERT",

author = "Park, {Hyun Hee} and Lee, {Kyu Yong} and Park, {Dong Woo} and Choi, {Na Young} and Lee, {Young Joo} and Son, {Jeong Woo} and Sangjae Kim and Chanil Moon and Kim, {Hyun Wook} and Rhyu, {Im Joo} and Koh, {Seong Ho}",

note = "Funding Information: The authors would like to thank Dr. Joseph A. Frank for his valuable comments and suggestions that have helped improve our work and the manuscript. This research was supported by the Basic Science Research Program of the National Research Foundation of Korea , which is funded by the Ministry of Science, ICT and Future Planning ( 2015R1A2A2A04004865 and 2015R1D1A1A01060644 ), by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) , funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI17C2160 ), by Korea Drug Development Fund ( KDDF ) funded by Ministry of Science and ICT, Ministry of Trade, Industry & Energy and Ministry of Health & Welfare ( KDDF-201609-02 , Republic of Korea), by and the Medical Research Center ( 2017R1A5A2015395 ). Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

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doi = "10.1016/j.biomaterials.2017.11.009",

language = "English",

volume = "155",

pages = "80--91",

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T1 - Tracking and protection of transplanted stem cells using a ferrocenecarboxylic acid-conjugated peptide that mimics hTERT

AU - Park, Hyun Hee

AU - Lee, Kyu Yong

AU - Park, Dong Woo

AU - Choi, Na Young

AU - Lee, Young Joo

AU - Son, Jeong Woo

AU - Kim, Sangjae

AU - Moon, Chanil

AU - Kim, Hyun Wook

AU - Rhyu, Im Joo

AU - Koh, Seong Ho

N1 - Funding Information: The authors would like to thank Dr. Joseph A. Frank for his valuable comments and suggestions that have helped improve our work and the manuscript. This research was supported by the Basic Science Research Program of the National Research Foundation of Korea , which is funded by the Ministry of Science, ICT and Future Planning ( 2015R1A2A2A04004865 and 2015R1D1A1A01060644 ), by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) , funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI17C2160 ), by Korea Drug Development Fund ( KDDF ) funded by Ministry of Science and ICT, Ministry of Trade, Industry & Energy and Ministry of Health & Welfare ( KDDF-201609-02 , Republic of Korea), by and the Medical Research Center ( 2017R1A5A2015395 ). Publisher Copyright: © 2017 Elsevier Ltd

PY - 2018/2

Y1 - 2018/2

N2 - In vivo tracking of transplanted stem cells has been a central aim of stem cell therapy. Although many tracking systems have been introduced, no method has yet been validated for clinical applications. We developed a novel sophisticated peptide (GV1001) that mimics hTERT (human telomerase reverse transcriptase) and analysed its ability to track and protect stem cells after transplantation. Ferrocenecarboxylic acid-conjugated GV1001 (Fe-GV1001) efficiently penetrated stem cells with no adverse effects. Moreover, Fe-GV1001 improved the viability, proliferation, and migration of stem cells under hypoxia. After Fe-GV1001-labelled stem cells were transplanted into the brains of rats after stroke, the labelled cells were easily tracked by MRI. Our findings indicate that Fe-GV1001 can be used for the in vivo tracking of stem cells after transplantation into the brain and can improve the efficacy of stem cell therapy by sustaining and enhancing stem cell characteristics under disease conditions.

AB - In vivo tracking of transplanted stem cells has been a central aim of stem cell therapy. Although many tracking systems have been introduced, no method has yet been validated for clinical applications. We developed a novel sophisticated peptide (GV1001) that mimics hTERT (human telomerase reverse transcriptase) and analysed its ability to track and protect stem cells after transplantation. Ferrocenecarboxylic acid-conjugated GV1001 (Fe-GV1001) efficiently penetrated stem cells with no adverse effects. Moreover, Fe-GV1001 improved the viability, proliferation, and migration of stem cells under hypoxia. After Fe-GV1001-labelled stem cells were transplanted into the brains of rats after stroke, the labelled cells were easily tracked by MRI. Our findings indicate that Fe-GV1001 can be used for the in vivo tracking of stem cells after transplantation into the brain and can improve the efficacy of stem cell therapy by sustaining and enhancing stem cell characteristics under disease conditions.

KW - MRI

KW - Mesenchymal stem cells

KW - Neural stem cells

KW - Peptide

KW - Tracking

KW - hTERT

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U2 - 10.1016/j.biomaterials.2017.11.009

DO - 10.1016/j.biomaterials.2017.11.009

M3 - Article

C2 - 29169040

AN - SCOPUS:85034421511

SN - 0142-9612

VL - 155

SP - 80

EP - 91

JO - Biomaterials

JF - Biomaterials

ER -

Tracking and protection of transplanted stem cells using a ferrocenecarboxylic acid-conjugated peptide that mimics hTERT

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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