Tracking and protection of transplanted stem cells using a ferrocenecarboxylic acid-conjugated peptide that mimics hTERT

Hyun Hee Park, Kyu Yong Lee, Dong Woo Park, Na Young Choi, Young Joo Lee, Jeong Woo Son, Sangjae Kim, Chanil Moon, Hyun Wook Kim, Im Joo Rhyu, Seong Ho Koh

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

In vivo tracking of transplanted stem cells has been a central aim of stem cell therapy. Although many tracking systems have been introduced, no method has yet been validated for clinical applications. We developed a novel sophisticated peptide (GV1001) that mimics hTERT (human telomerase reverse transcriptase) and analysed its ability to track and protect stem cells after transplantation. Ferrocenecarboxylic acid-conjugated GV1001 (Fe-GV1001) efficiently penetrated stem cells with no adverse effects. Moreover, Fe-GV1001 improved the viability, proliferation, and migration of stem cells under hypoxia. After Fe-GV1001-labelled stem cells were transplanted into the brains of rats after stroke, the labelled cells were easily tracked by MRI. Our findings indicate that Fe-GV1001 can be used for the in vivo tracking of stem cells after transplantation into the brain and can improve the efficacy of stem cell therapy by sustaining and enhancing stem cell characteristics under disease conditions.

Original languageEnglish
Pages (from-to)80-91
Number of pages12
JournalBiomaterials
Volume155
DOIs
Publication statusPublished - 2018 Feb

Bibliographical note

Funding Information:
The authors would like to thank Dr. Joseph A. Frank for his valuable comments and suggestions that have helped improve our work and the manuscript. This research was supported by the Basic Science Research Program of the National Research Foundation of Korea , which is funded by the Ministry of Science, ICT and Future Planning ( 2015R1A2A2A04004865 and 2015R1D1A1A01060644 ), by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) , funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI17C2160 ), by Korea Drug Development Fund ( KDDF ) funded by Ministry of Science and ICT, Ministry of Trade, Industry & Energy and Ministry of Health & Welfare ( KDDF-201609-02 , Republic of Korea), by and the Medical Research Center ( 2017R1A5A2015395 ).

Publisher Copyright:
© 2017 Elsevier Ltd

Keywords

  • MRI
  • Mesenchymal stem cells
  • Neural stem cells
  • Peptide
  • Tracking
  • hTERT

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Ceramics and Composites
  • Biomaterials
  • Mechanics of Materials

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