Transcriptional coactivator with PDZ-binding motif stimulates epidermal regeneration via induction of amphiregulin expression after ultraviolet damage

Kyung Min Kim; Ho Taek Oh; Gi Don Yoo; Jun Ha Hwang; Areum Oh; Eun Sook Hwang; Jeong Ho Hong

doi:10.1016/j.bbrc.2020.01.079

Transcriptional coactivator with PDZ-binding motif stimulates epidermal regeneration via induction of amphiregulin expression after ultraviolet damage

Kyung Min Kim, Ho Taek Oh, Gi Don Yoo, Jun Ha Hwang, Areum Oh, Eun Sook Hwang, Jeong Ho Hong

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Ultraviolet (UV) irradiation induces the proliferation and differentiation of keratinocytes in the basal layer of the epidermis, which increases epidermal thickness in skin regeneration. However, the mechanism underlying this phenomenon is not yet known in detail. In this study, we aimed to demonstrate that the transcriptional coactivator with PDZ-binding motif (TAZ) stimulates epidermal regeneration by increasing keratinocyte proliferation. During epidermal regeneration, TAZ is localized in the nucleus of keratinocytes of the basal layer and stimulates epidermal growth factor receptor (EGFR) signaling. TAZ depletion in keratinocytes decreased EGFR signaling activation, which delays epidermal regeneration. Interestingly, TAZ stimulated the transcription of amphiregulin (AREG), a ligand of EGFR, through TEAD-mediated transcriptional activation. Together, these results show that TAZ stimulates EGFR signaling through AREG induction, suggesting that it plays an important role in epidermal regeneration.

Original language	English
Pages (from-to)	242-248
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	524
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2020.01.079
Publication status	Published - 2020 Mar 26

Bibliographical note

Funding Information:
This work was supported by the Basic Science Research Program of the National Research Foundation (grant numbers 2019R1A2C2008430, 2015R1A5A1009024, and 2018R1A5A2025286) and grants of Ewha Education & Research Center for Infection (201900650001) and Korea University, Republic of Korea.

Funding Information:
This work was supported by the Basic Science Research Program of the National Research Foundation (grant numbers 2019R1A2C2008430 , 2015R1A5A1009024 , and 2018R1A5A2025286 ) and grants of Ewha Education & Research Center for Infection ( 201900650001 ) and Korea University, Republic of Korea .

Publisher Copyright:
© 2020 Elsevier Inc.

Keywords

AREG
EGFR signal
Skin regeneration
UV damage

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2020.01.079

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title = "Transcriptional coactivator with PDZ-binding motif stimulates epidermal regeneration via induction of amphiregulin expression after ultraviolet damage",

abstract = "Ultraviolet (UV) irradiation induces the proliferation and differentiation of keratinocytes in the basal layer of the epidermis, which increases epidermal thickness in skin regeneration. However, the mechanism underlying this phenomenon is not yet known in detail. In this study, we aimed to demonstrate that the transcriptional coactivator with PDZ-binding motif (TAZ) stimulates epidermal regeneration by increasing keratinocyte proliferation. During epidermal regeneration, TAZ is localized in the nucleus of keratinocytes of the basal layer and stimulates epidermal growth factor receptor (EGFR) signaling. TAZ depletion in keratinocytes decreased EGFR signaling activation, which delays epidermal regeneration. Interestingly, TAZ stimulated the transcription of amphiregulin (AREG), a ligand of EGFR, through TEAD-mediated transcriptional activation. Together, these results show that TAZ stimulates EGFR signaling through AREG induction, suggesting that it plays an important role in epidermal regeneration.",

keywords = "AREG, EGFR signal, Skin regeneration, UV damage",

author = "Kim, {Kyung Min} and Oh, {Ho Taek} and Yoo, {Gi Don} and Hwang, {Jun Ha} and Areum Oh and Hwang, {Eun Sook} and Hong, {Jeong Ho}",

note = "Funding Information: This work was supported by the Basic Science Research Program of the National Research Foundation (grant numbers 2019R1A2C2008430, 2015R1A5A1009024, and 2018R1A5A2025286) and grants of Ewha Education & Research Center for Infection (201900650001) and Korea University, Republic of Korea. Funding Information: This work was supported by the Basic Science Research Program of the National Research Foundation (grant numbers 2019R1A2C2008430 , 2015R1A5A1009024 , and 2018R1A5A2025286 ) and grants of Ewha Education & Research Center for Infection ( 201900650001 ) and Korea University, Republic of Korea . Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",

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AU - Kim, Kyung Min

AU - Oh, Ho Taek

AU - Yoo, Gi Don

AU - Hwang, Jun Ha

AU - Oh, Areum

AU - Hwang, Eun Sook

AU - Hong, Jeong Ho

N1 - Funding Information: This work was supported by the Basic Science Research Program of the National Research Foundation (grant numbers 2019R1A2C2008430, 2015R1A5A1009024, and 2018R1A5A2025286) and grants of Ewha Education & Research Center for Infection (201900650001) and Korea University, Republic of Korea. Funding Information: This work was supported by the Basic Science Research Program of the National Research Foundation (grant numbers 2019R1A2C2008430 , 2015R1A5A1009024 , and 2018R1A5A2025286 ) and grants of Ewha Education & Research Center for Infection ( 201900650001 ) and Korea University, Republic of Korea . Publisher Copyright: © 2020 Elsevier Inc.

PY - 2020/3/26

Y1 - 2020/3/26

N2 - Ultraviolet (UV) irradiation induces the proliferation and differentiation of keratinocytes in the basal layer of the epidermis, which increases epidermal thickness in skin regeneration. However, the mechanism underlying this phenomenon is not yet known in detail. In this study, we aimed to demonstrate that the transcriptional coactivator with PDZ-binding motif (TAZ) stimulates epidermal regeneration by increasing keratinocyte proliferation. During epidermal regeneration, TAZ is localized in the nucleus of keratinocytes of the basal layer and stimulates epidermal growth factor receptor (EGFR) signaling. TAZ depletion in keratinocytes decreased EGFR signaling activation, which delays epidermal regeneration. Interestingly, TAZ stimulated the transcription of amphiregulin (AREG), a ligand of EGFR, through TEAD-mediated transcriptional activation. Together, these results show that TAZ stimulates EGFR signaling through AREG induction, suggesting that it plays an important role in epidermal regeneration.

AB - Ultraviolet (UV) irradiation induces the proliferation and differentiation of keratinocytes in the basal layer of the epidermis, which increases epidermal thickness in skin regeneration. However, the mechanism underlying this phenomenon is not yet known in detail. In this study, we aimed to demonstrate that the transcriptional coactivator with PDZ-binding motif (TAZ) stimulates epidermal regeneration by increasing keratinocyte proliferation. During epidermal regeneration, TAZ is localized in the nucleus of keratinocytes of the basal layer and stimulates epidermal growth factor receptor (EGFR) signaling. TAZ depletion in keratinocytes decreased EGFR signaling activation, which delays epidermal regeneration. Interestingly, TAZ stimulated the transcription of amphiregulin (AREG), a ligand of EGFR, through TEAD-mediated transcriptional activation. Together, these results show that TAZ stimulates EGFR signaling through AREG induction, suggesting that it plays an important role in epidermal regeneration.

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Transcriptional coactivator with PDZ-binding motif stimulates epidermal regeneration via induction of amphiregulin expression after ultraviolet damage

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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