Transcriptomic analysis of Corynebacterium glutamicum in the response to the toxicity of furfural present in lignocellulosic hydrolysates

Hong Sil Park, Youngsoon Um, Sang Jun Sim, Sang Yup Lee, Han Min Woo

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Pretreatment of lignocellulosic biomass by acid hydrolysis generates growth inhibitors such as furfural, 5-hydroxymethylfurfural, and acetic acid. Among the inhibitors, furfural strongly inhibits cell growth, our objective was to identify the furfural stimulon of an amino acid producer Corynebacterium glutamicum using transcriptomic analysis. As a result, 182 up-regulated (2-fold) genes and 81 down-regulated (0.5-fold) genes were identified for all furfural stress conditions (6.5 mM, 13 mM, and 20 mM). Based on the functional categories of CoryneRegNet database, the furfural stimulon was related to genetic responses of oxidative stress, SOS responses, and anaerobic respiratory metabolism. To overcome the furfural toxicity, furfural-responsive efflux-like permease encoded by the cg1661 gene was overexpressed, resulting that the optical density at 600 nm was 1.54-fold enhanced over the control at 12 h. This study provides alternative strategy of metabolic engineering for biological detoxification in addition to overexpression of NADH-dependent reductases.

Original languageEnglish
Pages (from-to)347-356
Number of pages10
JournalProcess Biochemistry
Issue number3
Publication statusPublished - 2015 Mar

Bibliographical note

Funding Information:
Authors thank Prof. Michael Bott for the kind gift of pAN6. This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (Ministry of Science, ICT & Future Planning) (2014, University-Institute Cooperation program) and Creative Allied Program (CAP) of the Korea Research Council of Fundamental Science and Technology (KRCF)/Korea Institute of Science and Technology (KIST) (Project No. 2E24832). SYL was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the MSIP (NRF-2012-C1AAA001-2012M1A2A2026556).

Publisher Copyright:
© 2014 Elsevier Ltd. All rights reserved.

Copyright 2015 Elsevier B.V., All rights reserved.


  • Corynebacterium glutamicum
  • DNA microarray
  • Efflux-like permease
  • Furfural
  • Metabolic engineering

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology


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