Transcriptomic study for screening genes involved in the oxidative bioconversions of Streptomyces avermitilis

Hyo Jeong Kim, Kwon Young Choi, Da Hye Jung, Joon Young Jung, Eun Ok Jung, Yung Hun Yang, Byung Gee Kim, Min Kyu Oh

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Streptomyces avermitilis is a well known organism producing avermectin antibiotics, and has been utilized as an industrial host for oxidation bioconversion processes. Recently, gene screening strategies related to bioconversions have received much focus, as attempts are made to optimize oxidation and biodegradation pathways to maximize yield and productivity. Here, we have demonstrated the oxidative metabolisms of three molecules, daidzein, p-coumaric acid and mevastatin, where S. aver-mitilis converted each substrate to 3′,4′,7-trihydroxyisof-lavone, caffeic acid and hydroxyl-mevastatin to yield 9.3, 32.5 and 15.0 %, respectively. Microarray technology was exploited to investigate genome-wide analysis of gene expression changes, which were induced upon the addition of each substrate. Cytochrome P450 hydroxylases (pteC, cyp28 and olmB), diooxygenases (xylE, cdo1 and putatives) and LuxAB-like oxygenase were identified. One of them, cyp28, was indeed a gene encoding P450 hydroxylase responsible for the oxidative reaction of daidzein. Furthermore, possible electron transfer chain (fdrC → pteE → pteC) supporting cytochrome P450 dependent hydroxylation of daidzein has been suggested based on the interpretation of expression profiles. The result provided a potential application of transcriptomic study on uncovering enzymes involved in oxidative bioconversions of S. avermitilis.

Original languageEnglish
Pages (from-to)1621-1630
Number of pages10
JournalBioprocess and Biosystems Engineering
Issue number11
Publication statusPublished - 2013 Nov

Bibliographical note

Funding Information:
Acknowledgments The authors wish to acknowledge the financial support of the Seoul R&BD Program (KU080657) and WCU (World Class University) program through the National Research Foundation of Korea (NRF) grant funded by MEST (R322012000102130) and also through Institute of Bioengineering, Seoul National University, Seoul, Korea.


  • Biotransformation
  • Daidzein
  • Streptomyces avermitilis
  • Transcriptomics

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering


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