Transmission and regulation of biochemical stimulus via a nanoshell directly adsorbed on the cell membrane to enhance chondrogenic differentiation of mesenchymal stem cell

Uiyoung Han; Jun Ha Hwang; Jong Min Lee; Hyeoni Kim; Han Sung Jung; Jeong Ho Hong; Jinkee Hong

doi:10.1002/bit.27183

Transmission and regulation of biochemical stimulus via a nanoshell directly adsorbed on the cell membrane to enhance chondrogenic differentiation of mesenchymal stem cell

Uiyoung Han, Jun Ha Hwang, Jong Min Lee, Hyeoni Kim, Han Sung Jung, Jeong Ho Hong, Jinkee Hong

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

A nanoscale artificial extracellular matrix (nanoshell) formed by layer-by-layer adsorption can enhance and modulate the function of stem cells by transferring biochemical stimulus to the cell directly. Here, the nanoshell composed of fibronectin (FN) and chondroitin sulfate (CS) is demonstrated to promote chondrogenic differentiation of mesenchymal stem cells (MSCs). The multilayer structure of nanoshell is formed by repeating self-assembly of FN and CS, and its thickness can be controlled through the number of layers. The expression of chondrogenic markers in MSCs coated with the FN/CS nanoshell was increased as the number of bilayers in the nanoshell increased until four, but when it exceeds five bilayers, the effect began to decrease. Finally, the MSCs coated with optimized four bilayers of FN/CS nanoshell have high chondrogenic differentiation efficiency and showed the potential to increase formation of cartilage tissue when it is transplanted into mouse kidney. So, the precise regulation of stem cell fate at single cell level can be possible through the cellular surface modification by self-assembled polymeric film.

Original language	English
Pages (from-to)	184-193
Number of pages	10
Journal	Biotechnology and Bioengineering
Volume	117
Issue number	1
DOIs	https://doi.org/10.1002/bit.27183
Publication status	Published - 2020 Jan 1

Bibliographical note

Funding Information:
This study was also supported by the National Research Foundation of Korea (NRF) Grant funded by the Ministry of Science and ICT for First-Mover Program for Accelerating Disruptive Technology Development (NRF-2018M3C1B9066755). This study was also supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2017R1E1A1A01074343). In addition, this study was also supported by a grant from Korea Health Technology R&D Project [HI14C-3266].

Publisher Copyright:
© 2019 Wiley Periodicals, Inc.

Keywords

chondrogenic differentiation
extracellular matrix
mesenchymal stem cell
polymeric nanofilm

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1002/bit.27183

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Transmission and regulation of biochemical stimulus via a nanoshell directly adsorbed on the cell membrane to enhance chondrogenic differentiation of mesenchymal stem cell. / Han, Uiyoung; Hwang, Jun Ha; Lee, Jong Min et al.
In: Biotechnology and Bioengineering, Vol. 117, No. 1, 01.01.2020, p. 184-193.

Research output: Contribution to journal › Article › peer-review

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abstract = "A nanoscale artificial extracellular matrix (nanoshell) formed by layer-by-layer adsorption can enhance and modulate the function of stem cells by transferring biochemical stimulus to the cell directly. Here, the nanoshell composed of fibronectin (FN) and chondroitin sulfate (CS) is demonstrated to promote chondrogenic differentiation of mesenchymal stem cells (MSCs). The multilayer structure of nanoshell is formed by repeating self-assembly of FN and CS, and its thickness can be controlled through the number of layers. The expression of chondrogenic markers in MSCs coated with the FN/CS nanoshell was increased as the number of bilayers in the nanoshell increased until four, but when it exceeds five bilayers, the effect began to decrease. Finally, the MSCs coated with optimized four bilayers of FN/CS nanoshell have high chondrogenic differentiation efficiency and showed the potential to increase formation of cartilage tissue when it is transplanted into mouse kidney. So, the precise regulation of stem cell fate at single cell level can be possible through the cellular surface modification by self-assembled polymeric film.",

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note = "Funding Information: This study was also supported by the National Research Foundation of Korea (NRF) Grant funded by the Ministry of Science and ICT for First-Mover Program for Accelerating Disruptive Technology Development (NRF-2018M3C1B9066755). This study was also supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2017R1E1A1A01074343). In addition, this study was also supported by a grant from Korea Health Technology R&D Project [HI14C-3266]. Publisher Copyright: {\textcopyright} 2019 Wiley Periodicals, Inc.",

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Transmission and regulation of biochemical stimulus via a nanoshell directly adsorbed on the cell membrane to enhance chondrogenic differentiation of mesenchymal stem cell

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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