Transthyretin Is a Key Regulator of Myoblast Differentiation

Eun Ju Lee; Abdul R. Bhat; Majid Rasool Kamli; Smritee Pokharel; Tahoon Chun; Yong Ho Lee; Sang Seop Nahm; Joo Hyun Nam; Seong Koo Hong; Bohsuk Yang; Ki Young Chung; Sang Hoon Kim; Inho Choi

doi:10.1371/journal.pone.0063627

Transthyretin Is a Key Regulator of Myoblast Differentiation

Eun Ju Lee, Abdul R. Bhat, Majid Rasool Kamli, Smritee Pokharel, Tahoon Chun, Yong Ho Lee, Sang Seop Nahm, Joo Hyun Nam, Seong Koo Hong, Bohsuk Yang, Ki Young Chung, Sang Hoon Kim, Inho Choi

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Transthyretin (TTR) is a known carrier protein for thyroxine (T₄) and retinol-binding protein in the blood that is primarily synthesized in the liver and choroid plexus of the brain. Herein, we report that the TTR gene is expressed in skeletal muscle tissue and up-regulated during myotube formation in C2C12 cells. TTR silencing (TTR_kd) significantly reduced myogenin expression and myotube formation, whereas myogenin silencing (MYOG_kd) did not have any effect on TTR gene expression. Both TTR_kd and MYOG_kd led to a decrease in calcium channel related genes including Cav1.1, STIM1 and Orai1. A significant decrease in intracellular T₄ uptake during myogenesis was observed in TTR_kd cells. Taken together, the results of this study suggest that TTR initiates myoblast differentiation via affecting expression of the genes involved during early stage of myogenesis and the genes related to calcium channel.

Original language	English
Article number	e63627
Journal	PloS one
Volume	8
Issue number	5
DOIs	https://doi.org/10.1371/journal.pone.0063627
Publication status	Published - 2013 May 22

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title = "Transthyretin Is a Key Regulator of Myoblast Differentiation",

abstract = "Transthyretin (TTR) is a known carrier protein for thyroxine (T4) and retinol-binding protein in the blood that is primarily synthesized in the liver and choroid plexus of the brain. Herein, we report that the TTR gene is expressed in skeletal muscle tissue and up-regulated during myotube formation in C2C12 cells. TTR silencing (TTRkd) significantly reduced myogenin expression and myotube formation, whereas myogenin silencing (MYOGkd) did not have any effect on TTR gene expression. Both TTRkd and MYOGkd led to a decrease in calcium channel related genes including Cav1.1, STIM1 and Orai1. A significant decrease in intracellular T4 uptake during myogenesis was observed in TTRkd cells. Taken together, the results of this study suggest that TTR initiates myoblast differentiation via affecting expression of the genes involved during early stage of myogenesis and the genes related to calcium channel.",

author = "Lee, {Eun Ju} and Bhat, {Abdul R.} and Kamli, {Majid Rasool} and Smritee Pokharel and Tahoon Chun and Lee, {Yong Ho} and Nahm, {Sang Seop} and Nam, {Joo Hyun} and Hong, {Seong Koo} and Bohsuk Yang and Chung, {Ki Young} and Kim, {Sang Hoon} and Inho Choi",

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AU - Lee, Eun Ju

AU - Bhat, Abdul R.

AU - Kamli, Majid Rasool

AU - Pokharel, Smritee

AU - Chun, Tahoon

AU - Lee, Yong Ho

AU - Nahm, Sang Seop

AU - Nam, Joo Hyun

AU - Hong, Seong Koo

AU - Yang, Bohsuk

AU - Chung, Ki Young

AU - Kim, Sang Hoon

AU - Choi, Inho

PY - 2013/5/22

Y1 - 2013/5/22

N2 - Transthyretin (TTR) is a known carrier protein for thyroxine (T4) and retinol-binding protein in the blood that is primarily synthesized in the liver and choroid plexus of the brain. Herein, we report that the TTR gene is expressed in skeletal muscle tissue and up-regulated during myotube formation in C2C12 cells. TTR silencing (TTRkd) significantly reduced myogenin expression and myotube formation, whereas myogenin silencing (MYOGkd) did not have any effect on TTR gene expression. Both TTRkd and MYOGkd led to a decrease in calcium channel related genes including Cav1.1, STIM1 and Orai1. A significant decrease in intracellular T4 uptake during myogenesis was observed in TTRkd cells. Taken together, the results of this study suggest that TTR initiates myoblast differentiation via affecting expression of the genes involved during early stage of myogenesis and the genes related to calcium channel.

AB - Transthyretin (TTR) is a known carrier protein for thyroxine (T4) and retinol-binding protein in the blood that is primarily synthesized in the liver and choroid plexus of the brain. Herein, we report that the TTR gene is expressed in skeletal muscle tissue and up-regulated during myotube formation in C2C12 cells. TTR silencing (TTRkd) significantly reduced myogenin expression and myotube formation, whereas myogenin silencing (MYOGkd) did not have any effect on TTR gene expression. Both TTRkd and MYOGkd led to a decrease in calcium channel related genes including Cav1.1, STIM1 and Orai1. A significant decrease in intracellular T4 uptake during myogenesis was observed in TTRkd cells. Taken together, the results of this study suggest that TTR initiates myoblast differentiation via affecting expression of the genes involved during early stage of myogenesis and the genes related to calcium channel.

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JO - PLoS One

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IS - 5

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ER -

Transthyretin Is a Key Regulator of Myoblast Differentiation

Abstract

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