Trophoblast glycoprotein is a marker for efficient sorting of ventral mesencephalic dopaminergic precursors derived from human pluripotent stem cells

Jeong Eun Yoo, Dongjin R. Lee, Sanghyun Park, Hye Rim Shin, Kun Gu Lee, Dae Sung Kim, Mi Young Jo, Jang Hyeon Eom, Myung Soo Cho, Dong Youn Hwang, Dong Wook Kim

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Successful cell therapy for Parkinson’s disease (PD) requires large numbers of homogeneous ventral mesencephalic dopaminergic (vmDA) precursors. Enrichment of vmDA precursors via cell sorting is required to ensure high safety and efficacy of the cell therapy. Here, using LMX1A-eGFP knock-in reporter human embryonic stem cells, we discovered a novel surface antigen, trophoblast glycoprotein (TPBG), which was preferentially expressed in vmDA precursors. TPBG-targeted cell sorting enriched FOXA2+LMX1A+ vmDA precursors and helped attain efficient behavioral recovery of rodent PD models with increased numbers of TH+, NURR1+, and PITX3+ vmDA neurons in the grafts. Additionally, fewer proliferating cells were detected in TPBG+ cell-derived grafts than in TPBG cell-derived grafts. Our approach is an efficient way to obtain enriched bona fide vmDA precursors, which could open a new avenue for effective PD treatment.

Original languageEnglish
Article number61
Journalnpj Parkinson's Disease
Volume7
Issue number1
DOIs
Publication statusPublished - 2021 Dec

Bibliographical note

Funding Information:
D.-W.K. was supported by the Korea Health Technology R&D Project Grant (HI18C0829) through the Korea Health Industry Development Institute, founded by the Ministry of Health & Welfare. D.-Y.H. and M.S.C. were supported by a grant (HI18C0096) from the Ministry of Health and Welfare. This study was carried out in part in the Yonsei Advanced Imaging Center in cooperation with Carl Zeiss Microscopy, Yonsei University College of Medicine.

Publisher Copyright:
© 2021, The Author(s).

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

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