TRP-independent inhibition of the phospholipase C pathway by natural sensory ligands

Kyung Yoon Kim; Sangsu Bang; Shanshu Han; Yen Hoang Nguyen; Tong Mook Kang; Keon Wook Kang; Sun Wook Hwang

doi:10.1016/j.bbrc.2008.03.077

TRP-independent inhibition of the phospholipase C pathway by natural sensory ligands

Kyung Yoon Kim, Sangsu Bang, Shanshu Han, Yen Hoang Nguyen, Tong Mook Kang, Keon Wook Kang^*, Sun Wook Hwang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Menthol, cinnamaldehyde, and camphor are activators for temperature-sensitive transient receptor potential ion channels (thermoTRPs). Here we found that these three compounds inhibit the phospholipase C (PLC) signaling. P2Y purinoceptor-mediated or histamine receptor-mediated cytosolic calcium mobilization through the PLC pathway was significantly suppressed by menthol, cinnamaldehyde, and camphor. Experiments using a fluorescent pleckstrin homology domain of PLCδ1 and IP1 accumulation assays demonstrated that direct inhibition of PLC activity occurred upon the addition of the sensory compounds. P2Y receptor-mediated PLC activation is part of the mechanism of platelet aggregation. The three compounds inhibited ADP-induced platelet aggregation. Calcium influx studies showed that thermoTRPs do not function in platelets, suggesting that the anti-aggregation effect is independent of thermoTRP activity. These results suggest that menthol, cinnamaldehyde, and camphor are able to modify PLC signaling and that those effects may lead to changes in cellular functions. This study also identifies new types of compounds that could potentially modulate platelet-related pathological events.

Original language	English
Pages (from-to)	295-300
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	370
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2008.03.077
Publication status	Published - 2008 May 30

Bibliographical note

Funding Information:
This research was supported by the Korea Research Foundation Grant (code KRF-2005-041-E00486), the Republic of Korea.

Keywords

Camphor
Cinnamaldehyde
Menthol
Phospholipase C
Platelet aggregation

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2008.03.077

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title = "TRP-independent inhibition of the phospholipase C pathway by natural sensory ligands",

abstract = "Menthol, cinnamaldehyde, and camphor are activators for temperature-sensitive transient receptor potential ion channels (thermoTRPs). Here we found that these three compounds inhibit the phospholipase C (PLC) signaling. P2Y purinoceptor-mediated or histamine receptor-mediated cytosolic calcium mobilization through the PLC pathway was significantly suppressed by menthol, cinnamaldehyde, and camphor. Experiments using a fluorescent pleckstrin homology domain of PLCδ1 and IP1 accumulation assays demonstrated that direct inhibition of PLC activity occurred upon the addition of the sensory compounds. P2Y receptor-mediated PLC activation is part of the mechanism of platelet aggregation. The three compounds inhibited ADP-induced platelet aggregation. Calcium influx studies showed that thermoTRPs do not function in platelets, suggesting that the anti-aggregation effect is independent of thermoTRP activity. These results suggest that menthol, cinnamaldehyde, and camphor are able to modify PLC signaling and that those effects may lead to changes in cellular functions. This study also identifies new types of compounds that could potentially modulate platelet-related pathological events.",

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author = "Kim, \{Kyung Yoon\} and Sangsu Bang and Shanshu Han and Nguyen, \{Yen Hoang\} and Kang, \{Tong Mook\} and Kang, \{Keon Wook\} and Hwang, \{Sun Wook\}",

note = "Funding Information: This research was supported by the Korea Research Foundation Grant (code KRF-2005-041-E00486), the Republic of Korea.",

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doi = "10.1016/j.bbrc.2008.03.077",

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AU - Kim, Kyung Yoon

AU - Bang, Sangsu

AU - Han, Shanshu

AU - Nguyen, Yen Hoang

AU - Kang, Tong Mook

AU - Kang, Keon Wook

AU - Hwang, Sun Wook

N1 - Funding Information: This research was supported by the Korea Research Foundation Grant (code KRF-2005-041-E00486), the Republic of Korea.

PY - 2008/5/30

Y1 - 2008/5/30

N2 - Menthol, cinnamaldehyde, and camphor are activators for temperature-sensitive transient receptor potential ion channels (thermoTRPs). Here we found that these three compounds inhibit the phospholipase C (PLC) signaling. P2Y purinoceptor-mediated or histamine receptor-mediated cytosolic calcium mobilization through the PLC pathway was significantly suppressed by menthol, cinnamaldehyde, and camphor. Experiments using a fluorescent pleckstrin homology domain of PLCδ1 and IP1 accumulation assays demonstrated that direct inhibition of PLC activity occurred upon the addition of the sensory compounds. P2Y receptor-mediated PLC activation is part of the mechanism of platelet aggregation. The three compounds inhibited ADP-induced platelet aggregation. Calcium influx studies showed that thermoTRPs do not function in platelets, suggesting that the anti-aggregation effect is independent of thermoTRP activity. These results suggest that menthol, cinnamaldehyde, and camphor are able to modify PLC signaling and that those effects may lead to changes in cellular functions. This study also identifies new types of compounds that could potentially modulate platelet-related pathological events.

AB - Menthol, cinnamaldehyde, and camphor are activators for temperature-sensitive transient receptor potential ion channels (thermoTRPs). Here we found that these three compounds inhibit the phospholipase C (PLC) signaling. P2Y purinoceptor-mediated or histamine receptor-mediated cytosolic calcium mobilization through the PLC pathway was significantly suppressed by menthol, cinnamaldehyde, and camphor. Experiments using a fluorescent pleckstrin homology domain of PLCδ1 and IP1 accumulation assays demonstrated that direct inhibition of PLC activity occurred upon the addition of the sensory compounds. P2Y receptor-mediated PLC activation is part of the mechanism of platelet aggregation. The three compounds inhibited ADP-induced platelet aggregation. Calcium influx studies showed that thermoTRPs do not function in platelets, suggesting that the anti-aggregation effect is independent of thermoTRP activity. These results suggest that menthol, cinnamaldehyde, and camphor are able to modify PLC signaling and that those effects may lead to changes in cellular functions. This study also identifies new types of compounds that could potentially modulate platelet-related pathological events.

KW - Camphor

KW - Cinnamaldehyde

KW - Menthol

KW - Phospholipase C

KW - Platelet aggregation

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ER -

TRP-independent inhibition of the phospholipase C pathway by natural sensory ligands

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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