Abstract
TTF-1 is a member of the NKx family of homeodomain genes, and is required for morphogenesis and fetal diencephalon development. Our previous studies have shown that TTF-1 expression is maintained in some regions of the postnatal rat brain and transactivates the gene expression of several neuropeptides. In this study, a potential role for TTF-1 in the regulation of feeding behavior was identified. Immunohistochemical analysis showed that TTF-1 is present in several hypothalamic nuclei of the adult rat brain involved in the control of feeding behavior. Food deprivation for two days markedly increased the hypothalamic levels of TTF-1 mRNA and protein. Intracerebroventricular administration of an antisense TTF-1 oligodeoxynucleotide significantly decreased TTF-1 protein abundance in the hypothalamus. This TTF-1 decrease was followed by a significant decrease in neuropeptide Y mRNA content and an increase in proopiomelanocortin mRNA content, and in turn resulted in a decrease of the animal's food intake and body weight. These results suggest a novel role for TTF-1 in the regulation of feeding behavior in the rat hypothalamus.
Original language | English |
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Pages (from-to) | 969-975 |
Number of pages | 7 |
Journal | Biochemical and biophysical research communications |
Volume | 349 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2006 Oct 27 |
Externally published | Yes |
Bibliographical note
Funding Information:This work was supported by the Korea Research Foundation Grant (KRF-2002-070-C00068). J.G.K. and C.H.Y. were partially supported by Brain Korea 21 Grant (C119).
Keywords
- Food intake
- Hypothalamus
- Neuropeptide Y
- Obesity
- Proopiomelanocortin
- TTF-1
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology