TTG1-mediated flavonols biosynthesis alleviates root growth inhibition in response to ABA

Hoai Nguyen Nguyen; Jun Hyeok Kim; Woo Young Hyun; Ngoc Trinh Nguyen; Suk Whan Hong; Hojoung Lee

doi:10.1007/s00299-012-1382-1

TTG1-mediated flavonols biosynthesis alleviates root growth inhibition in response to ABA

Hoai Nguyen Nguyen, Jun Hyeok Kim, Woo Young Hyun, Ngoc Trinh Nguyen, Suk Whan Hong, Hojoung Lee

Department of Biosystems and Biotechnology

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Key message: Our results demonstrate that the flavonoids biosynthetic pathway can be effectively manipulated to confer enhanced plant root growth under water-stress conditions. Abscisic acid (ABA) is one of most important phytohormones. It functions in various processes during the plant lifecycle. Previous studies indicate that ABA has a negative effect on root growth and branching. Auxin is another key plant growth regulator that plays an essential role in plant growth and development. In contrast to ABA, auxin is a positive regulator of root growth and development at low concentrations. This study was performed to help understand whether flavonoids can suppress the effect of ABA on lateral root growth. The recessive TRANSPARENT TESTA GLABRA 1 (ttg1) mutant was characterized on ABA and sucrose treatments. It was determined that auxin mobilization could be altered by modifying flavonoids biosynthesis, which resulted in alterations of root architecture in response to ABA treatment. Moreover, transgenic TTG1-overexpression (TTG1-OX) seedlings exhibited enhanced root length and lateral root number compared to wild-type seedlings grown under normal or stress conditions. Genetic manipulation of the flavonoids biosynthetic pathway could therefore be employed successfully for the improvement of plant root systems by overcoming the inhibition of ABA and some abiotic stresses.

Original language	English
Pages (from-to)	503-514
Number of pages	12
Journal	Plant Cell Reports
Volume	32
Issue number	4
DOIs	https://doi.org/10.1007/s00299-012-1382-1
Publication status	Published - 2013 Apr

Keywords

ABA
Anthocyanin
Arabidopsis thaliana
Flavonoids
Root growth inhibition
TTG1

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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10.1007/s00299-012-1382-1

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title = "TTG1-mediated flavonols biosynthesis alleviates root growth inhibition in response to ABA",

abstract = "Key message: Our results demonstrate that the flavonoids biosynthetic pathway can be effectively manipulated to confer enhanced plant root growth under water-stress conditions. Abscisic acid (ABA) is one of most important phytohormones. It functions in various processes during the plant lifecycle. Previous studies indicate that ABA has a negative effect on root growth and branching. Auxin is another key plant growth regulator that plays an essential role in plant growth and development. In contrast to ABA, auxin is a positive regulator of root growth and development at low concentrations. This study was performed to help understand whether flavonoids can suppress the effect of ABA on lateral root growth. The recessive TRANSPARENT TESTA GLABRA 1 (ttg1) mutant was characterized on ABA and sucrose treatments. It was determined that auxin mobilization could be altered by modifying flavonoids biosynthesis, which resulted in alterations of root architecture in response to ABA treatment. Moreover, transgenic TTG1-overexpression (TTG1-OX) seedlings exhibited enhanced root length and lateral root number compared to wild-type seedlings grown under normal or stress conditions. Genetic manipulation of the flavonoids biosynthetic pathway could therefore be employed successfully for the improvement of plant root systems by overcoming the inhibition of ABA and some abiotic stresses.",

keywords = "ABA, Anthocyanin, Arabidopsis thaliana, Flavonoids, Root growth inhibition, TTG1",

author = "Nguyen, {Hoai Nguyen} and Kim, {Jun Hyeok} and Hyun, {Woo Young} and Nguyen, {Ngoc Trinh} and Hong, {Suk Whan} and Hojoung Lee",

note = "Funding Information: Acknowledgments This work was supported by a grant from the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (to Hojoung Lee, 2012; grant #2012-112068-3) and by a grant from the National Research Foundation (to Suk-Whan Hong; grant ##2012-0005857, #2012R1A1A4A01006448).",

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doi = "10.1007/s00299-012-1382-1",

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AU - Nguyen, Ngoc Trinh

AU - Hong, Suk Whan

AU - Lee, Hojoung

N1 - Funding Information: Acknowledgments This work was supported by a grant from the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (to Hojoung Lee, 2012; grant #2012-112068-3) and by a grant from the National Research Foundation (to Suk-Whan Hong; grant ##2012-0005857, #2012R1A1A4A01006448).

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N2 - Key message: Our results demonstrate that the flavonoids biosynthetic pathway can be effectively manipulated to confer enhanced plant root growth under water-stress conditions. Abscisic acid (ABA) is one of most important phytohormones. It functions in various processes during the plant lifecycle. Previous studies indicate that ABA has a negative effect on root growth and branching. Auxin is another key plant growth regulator that plays an essential role in plant growth and development. In contrast to ABA, auxin is a positive regulator of root growth and development at low concentrations. This study was performed to help understand whether flavonoids can suppress the effect of ABA on lateral root growth. The recessive TRANSPARENT TESTA GLABRA 1 (ttg1) mutant was characterized on ABA and sucrose treatments. It was determined that auxin mobilization could be altered by modifying flavonoids biosynthesis, which resulted in alterations of root architecture in response to ABA treatment. Moreover, transgenic TTG1-overexpression (TTG1-OX) seedlings exhibited enhanced root length and lateral root number compared to wild-type seedlings grown under normal or stress conditions. Genetic manipulation of the flavonoids biosynthetic pathway could therefore be employed successfully for the improvement of plant root systems by overcoming the inhibition of ABA and some abiotic stresses.

AB - Key message: Our results demonstrate that the flavonoids biosynthetic pathway can be effectively manipulated to confer enhanced plant root growth under water-stress conditions. Abscisic acid (ABA) is one of most important phytohormones. It functions in various processes during the plant lifecycle. Previous studies indicate that ABA has a negative effect on root growth and branching. Auxin is another key plant growth regulator that plays an essential role in plant growth and development. In contrast to ABA, auxin is a positive regulator of root growth and development at low concentrations. This study was performed to help understand whether flavonoids can suppress the effect of ABA on lateral root growth. The recessive TRANSPARENT TESTA GLABRA 1 (ttg1) mutant was characterized on ABA and sucrose treatments. It was determined that auxin mobilization could be altered by modifying flavonoids biosynthesis, which resulted in alterations of root architecture in response to ABA treatment. Moreover, transgenic TTG1-overexpression (TTG1-OX) seedlings exhibited enhanced root length and lateral root number compared to wild-type seedlings grown under normal or stress conditions. Genetic manipulation of the flavonoids biosynthetic pathway could therefore be employed successfully for the improvement of plant root systems by overcoming the inhibition of ABA and some abiotic stresses.

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KW - Anthocyanin

KW - Arabidopsis thaliana

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TTG1-mediated flavonols biosynthesis alleviates root growth inhibition in response to ABA

Abstract

Keywords

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