Tubby domain superfamily protein is required for the formation of the 7S SNARE complex in Drosophila

Eun Jang Yoon, Yong Taek Jeong, Ji Eun Lee, Seok Jun Moon, Chul Hoon Kim

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Tubby domain superfamily protein (TUSP) is a distant member of the Tubby-like protein (TULP) family. Although other TULPs play important roles in sensation, metabolism, and development, the molecular functions of TUSP are completely unknown. Here, we explore the function of TUSP in the Drosophila nervous system where it is expressed in all neurons. Tusp mutant flies exhibit a temperature-sensitive paralysis. This paralysis can be rescued by tissue-specific expression of Tusp in the giant fibers and peripherally synapsing interneurons of the giant fiber system, a well-characterized neuronal circuit that mediates rapid escape behavior in flies. Consistent with this paralytic phenotype, we observed a profound reduction in the assembly of the ternary 7S SNARE complex that is required for neurotransmitter release despite seeing no changes in the expression of each individual SNARE complex component. Together, these data suggest TUSP is a novel regulator of SNARE assembly and, therefore, of neurotransmitter release.

Original languageEnglish
Pages (from-to)814-820
Number of pages7
JournalBiochemical and biophysical research communications
Issue number4
Publication statusPublished - 2017 Jan 22
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Inc.


  • 7S SNARE complex
  • Drosophila
  • Giant fiber system
  • Temperature-sensitive paralysis
  • Tubby domain superfamily protein
  • Tulp4

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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