Two-color probe to monitor a wide range of pH values in cells

Min Hee Lee, Ji Hye Han, Jae Hong Lee, Nayoung Park, Rajesh Kumar, Chulhun Kang, Jong Seung Kim

Research output: Contribution to journalArticlepeer-review

231 Citations (Scopus)


High-tech pH paper: A "chameleon" pH probe composed of rhodamine (red, see scheme) and fluorescein (green) units emits at wavelengths of 580 nm and 512 nm, where the intensities show a contrary response to pH changes. Confocal microscopy of HeLa cells with this probe reveals red and green spots; the ratio of these signals can be calibrated to give the pH value of the respective organelle.

Original languageEnglish
Pages (from-to)6206-6209
Number of pages4
JournalAngewandte Chemie - International Edition
Issue number24
Publication statusPublished - 2013 Jun 10


  • fluorescein
  • fluorescent probes
  • imaging agents
  • intracellular pH
  • rhodamine

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry


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