Two-color probe to monitor a wide range of pH values in cells

Min Hee Lee; Ji Hye Han; Jae Hong Lee; Nayoung Park; Rajesh Kumar; Chulhun Kang; Jong Seung Kim

doi:10.1002/anie.201301894

Two-color probe to monitor a wide range of pH values in cells

Min Hee Lee
, Ji Hye Han
, Jae Hong Lee
, Nayoung Park
, Rajesh Kumar
, Chulhun Kang^*
, Jong Seung Kim

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

246 Citations (Scopus)

Abstract

High-tech pH paper: A "chameleon" pH probe composed of rhodamine (red, see scheme) and fluorescein (green) units emits at wavelengths of 580 nm and 512 nm, where the intensities show a contrary response to pH changes. Confocal microscopy of HeLa cells with this probe reveals red and green spots; the ratio of these signals can be calibrated to give the pH value of the respective organelle.

Original language	English
Pages (from-to)	6206-6209
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	52
Issue number	24
DOIs	https://doi.org/10.1002/anie.201301894
Publication status	Published - 2013 Jun 10

Keywords

fluorescein
fluorescent probes
imaging agents
intracellular pH
rhodamine

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.201301894

Cite this

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AU - Kumar, Rajesh

AU - Kang, Chulhun

AU - Kim, Jong Seung

PY - 2013/6/10

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AB - High-tech pH paper: A "chameleon" pH probe composed of rhodamine (red, see scheme) and fluorescein (green) units emits at wavelengths of 580 nm and 512 nm, where the intensities show a contrary response to pH changes. Confocal microscopy of HeLa cells with this probe reveals red and green spots; the ratio of these signals can be calibrated to give the pH value of the respective organelle.

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KW - fluorescent probes

KW - imaging agents

KW - intracellular pH

KW - rhodamine

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ER -

Two-color probe to monitor a wide range of pH values in cells

Abstract

Keywords

ASJC Scopus subject areas

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