Two combined amino acids promote sleep activity in caffeine-induced sleepless model systems

Ki Bae Hong, Yooheon Park, Hyung Joo Suh

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


BACKGROUND/OBJECTIVES: The aim of this study was to evaluate the biological and sleep-promoting effects of combined γ-aminobutyric acid (GABA) and 5-hydroxytryptophan (5-HTP) using caffeine-induced sleepless fruit flies, ICR mice, and Sprague-Dawley rats. MATERIALS/METHODS: Video-tracking analysis was applied to investigate behavioral changes of Drosophila melanogaster. Pentobarbital-induced sleep test and electroencephalogram (EEG) patterns were used for analysis of sleep latency, duration, and quantity and quality of sleep in vertebrate models. RESULTS: Administration of combined GABA/5-HTP could significantly reverse the caffeine induced total distance of flies (P < 0.001). Also, individually administered and combined GABA/5-HTP significantly increased the total sleeping time in the caffeine-induced sleepless ICR mice (P < 0.001). In the caffeine-induced sleepless SD-rats, combined GABA/5-HTP showed significant differences in sleep quality between individual amino acid administrations (P < 0.05). CONCLUSIONS: Taken together, we identified inhibitory effects of combined GABA/5-HTP in locomotor activity, sleep quantity and quality in caffeine-induced sleepless models, indicating that combined GABA/5-HTP may be effective in patients with insomnia by providing sufficient sleep.

Original languageEnglish
Pages (from-to)208-214
Number of pages7
JournalNutrition Research and Practice
Issue number3
Publication statusPublished - 2018 Jun


  • 5-HTP
  • Caffeine
  • GABA
  • Insomnia
  • Sleep

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics


Dive into the research topics of 'Two combined amino acids promote sleep activity in caffeine-induced sleepless model systems'. Together they form a unique fingerprint.

Cite this