Two-photon fluorescent probes for biomembrane imaging: effect of chain length.

Hwan Myung Kim, B. R. Kim, Hyo Jung Choo, Young Gyu Ko, Seung Joon Jeon, Chul Hoon Kim, Taiha Joo, Bong Rae Cho

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)


Two-photon fluorescent probes for the cellular membrane, derived from 6-acyl-2-aminonaphthalene as the fluorophore and hexanoyl (CH), lauryl (CL), and stearyl (CS) groups as the receptor, have been synthesized. Their photophysical properties and utility as membrane probes were also studied. Whereas CH cannot be used as a membrane probe due to its high water solubility, CL and CS are useful two-photon probes for membrane lateral heterogeneity, as they can easily stain cells, emit fluorescence with high sensitivity to the environment polarity, and are capable of imaging the membrane lateral heterogeneity in live cells. Moreover, CS is more likely to be located in the plasma membrane due to its negligible water solubility. Our results show that the liquid ordered-like domain covers 31-35% of the cellular surface.

Original languageEnglish
Pages (from-to)2830-2838
Number of pages9
JournalChembiochem : a European journal of chemical biology
Issue number17
Publication statusPublished - 2008 Nov 24

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry


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