TY - JOUR
T1 - Two-photon fluorescent probes for biomembrane imaging
T2 - effect of chain length.
AU - Kim, Hwan Myung
AU - Kim, B. R.
AU - Choo, Hyo Jung
AU - Ko, Young Gyu
AU - Jeon, Seung Joon
AU - Kim, Chul Hoon
AU - Joo, Taiha
AU - Cho, Bong Rae
PY - 2008/11/24
Y1 - 2008/11/24
N2 - Two-photon fluorescent probes for the cellular membrane, derived from 6-acyl-2-aminonaphthalene as the fluorophore and hexanoyl (CH), lauryl (CL), and stearyl (CS) groups as the receptor, have been synthesized. Their photophysical properties and utility as membrane probes were also studied. Whereas CH cannot be used as a membrane probe due to its high water solubility, CL and CS are useful two-photon probes for membrane lateral heterogeneity, as they can easily stain cells, emit fluorescence with high sensitivity to the environment polarity, and are capable of imaging the membrane lateral heterogeneity in live cells. Moreover, CS is more likely to be located in the plasma membrane due to its negligible water solubility. Our results show that the liquid ordered-like domain covers 31-35% of the cellular surface.
AB - Two-photon fluorescent probes for the cellular membrane, derived from 6-acyl-2-aminonaphthalene as the fluorophore and hexanoyl (CH), lauryl (CL), and stearyl (CS) groups as the receptor, have been synthesized. Their photophysical properties and utility as membrane probes were also studied. Whereas CH cannot be used as a membrane probe due to its high water solubility, CL and CS are useful two-photon probes for membrane lateral heterogeneity, as they can easily stain cells, emit fluorescence with high sensitivity to the environment polarity, and are capable of imaging the membrane lateral heterogeneity in live cells. Moreover, CS is more likely to be located in the plasma membrane due to its negligible water solubility. Our results show that the liquid ordered-like domain covers 31-35% of the cellular surface.
UR - http://www.scopus.com/inward/record.url?scp=58149394483&partnerID=8YFLogxK
U2 - 10.1002/cbic.200800353
DO - 10.1002/cbic.200800353
M3 - Article
C2 - 18973164
AN - SCOPUS:58149394483
SN - 1439-4227
VL - 9
SP - 2830
EP - 2838
JO - Chembiochem : a European journal of chemical biology
JF - Chembiochem : a European journal of chemical biology
IS - 17
ER -