Two-photon fluorescent probes for long-term imaging of calcium waves in live tissue

Hwan Myung Kim; Bo Ra Kim; Myoung Jin An; Jin Hee Hong; Kyoung J. Lee; Bong Rae Cho

doi:10.1002/chem.200701453

Two-photon fluorescent probes for long-term imaging of calcium waves in live tissue

Hwan Myung Kim, Bo Ra Kim, Myoung Jin An, Jin Hee Hong, Kyoung J. Lee, Bong Rae Cho

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

2-Acetyl-6-(dimethylamino)-naphthalene-derived two-photon fluorescent Ca²⁺ probes (ACa1-ACa3) are reported. They can be excited by a 780 nm laser beam, show 23-50-fold enhancement in one- and two-photon excited fluorescence in response to Ca²⁺, emit fourfold stronger two-photon excited fluorescence than Oregon Green 488 BAPTA-1 upon complexation with Ca²⁺, and can selectively detect intracellular free Ca²⁺ ions in live cells and living tissues with minimum interference from other metal ions and membrane-bound probes. Moreover, these probes are capable of monitoring calcium waves at a depth of 120-170 μm in live tissues for 1100-4000 s using two-photon microscopy with no artifacts of photobleaching.

Original language	English
Pages (from-to)	2075-2083
Number of pages	9
Journal	Chemistry - A European Journal
Volume	14
Issue number	7
DOIs	https://doi.org/10.1002/chem.200701453
Publication status	Published - 2008 Feb 27

Keywords

Calcium
Fluorescent probes
Imaging agents
Live tissue
Two-photon microscopy

ASJC Scopus subject areas

Catalysis
Organic Chemistry

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10.1002/chem.200701453

Cite this

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title = "Two-photon fluorescent probes for long-term imaging of calcium waves in live tissue",

abstract = "2-Acetyl-6-(dimethylamino)-naphthalene-derived two-photon fluorescent Ca2+ probes (ACa1-ACa3) are reported. They can be excited by a 780 nm laser beam, show 23-50-fold enhancement in one- and two-photon excited fluorescence in response to Ca2+, emit fourfold stronger two-photon excited fluorescence than Oregon Green 488 BAPTA-1 upon complexation with Ca2+, and can selectively detect intracellular free Ca2+ ions in live cells and living tissues with minimum interference from other metal ions and membrane-bound probes. Moreover, these probes are capable of monitoring calcium waves at a depth of 120-170 μm in live tissues for 1100-4000 s using two-photon microscopy with no artifacts of photobleaching.",

keywords = "Calcium, Fluorescent probes, Imaging agents, Live tissue, Two-photon microscopy",

author = "Kim, {Hwan Myung} and Kim, {Bo Ra} and An, {Myoung Jin} and Hong, {Jin Hee} and Lee, {Kyoung J.} and Cho, {Bong Rae}",

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T1 - Two-photon fluorescent probes for long-term imaging of calcium waves in live tissue

AU - Kim, Hwan Myung

AU - Kim, Bo Ra

AU - An, Myoung Jin

AU - Hong, Jin Hee

AU - Lee, Kyoung J.

AU - Cho, Bong Rae

PY - 2008/2/27

Y1 - 2008/2/27

N2 - 2-Acetyl-6-(dimethylamino)-naphthalene-derived two-photon fluorescent Ca2+ probes (ACa1-ACa3) are reported. They can be excited by a 780 nm laser beam, show 23-50-fold enhancement in one- and two-photon excited fluorescence in response to Ca2+, emit fourfold stronger two-photon excited fluorescence than Oregon Green 488 BAPTA-1 upon complexation with Ca2+, and can selectively detect intracellular free Ca2+ ions in live cells and living tissues with minimum interference from other metal ions and membrane-bound probes. Moreover, these probes are capable of monitoring calcium waves at a depth of 120-170 μm in live tissues for 1100-4000 s using two-photon microscopy with no artifacts of photobleaching.

AB - 2-Acetyl-6-(dimethylamino)-naphthalene-derived two-photon fluorescent Ca2+ probes (ACa1-ACa3) are reported. They can be excited by a 780 nm laser beam, show 23-50-fold enhancement in one- and two-photon excited fluorescence in response to Ca2+, emit fourfold stronger two-photon excited fluorescence than Oregon Green 488 BAPTA-1 upon complexation with Ca2+, and can selectively detect intracellular free Ca2+ ions in live cells and living tissues with minimum interference from other metal ions and membrane-bound probes. Moreover, these probes are capable of monitoring calcium waves at a depth of 120-170 μm in live tissues for 1100-4000 s using two-photon microscopy with no artifacts of photobleaching.

KW - Calcium

KW - Fluorescent probes

KW - Imaging agents

KW - Live tissue

KW - Two-photon microscopy

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DO - 10.1002/chem.200701453

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JO - Chemistry - A European Journal

JF - Chemistry - A European Journal

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Two-photon fluorescent probes for long-term imaging of calcium waves in live tissue

Abstract

Keywords

ASJC Scopus subject areas

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