Two-photon fluorescent probes for long-term imaging of calcium waves in live tissue

Hwan Myung Kim, Bo Ra Kim, Myoung Jin An, Jin Hee Hong, Kyoung J. Lee, Bong Rae Cho

    Research output: Contribution to journalArticlepeer-review

    50 Citations (Scopus)

    Abstract

    2-Acetyl-6-(dimethylamino)-naphthalene-derived two-photon fluorescent Ca2+ probes (ACa1-ACa3) are reported. They can be excited by a 780 nm laser beam, show 23-50-fold enhancement in one- and two-photon excited fluorescence in response to Ca2+, emit fourfold stronger two-photon excited fluorescence than Oregon Green 488 BAPTA-1 upon complexation with Ca2+, and can selectively detect intracellular free Ca2+ ions in live cells and living tissues with minimum interference from other metal ions and membrane-bound probes. Moreover, these probes are capable of monitoring calcium waves at a depth of 120-170 μm in live tissues for 1100-4000 s using two-photon microscopy with no artifacts of photobleaching.

    Original languageEnglish
    Pages (from-to)2075-2083
    Number of pages9
    JournalChemistry - A European Journal
    Volume14
    Issue number7
    DOIs
    Publication statusPublished - 2008 Feb 27

    Keywords

    • Calcium
    • Fluorescent probes
    • Imaging agents
    • Live tissue
    • Two-photon microscopy

    ASJC Scopus subject areas

    • General Chemistry
    • Catalysis
    • Organic Chemistry

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