Abstract
2-Acetyl-6-(dimethylamino)-naphthalene-derived two-photon fluorescent Ca2+ probes (ACa1-ACa3) are reported. They can be excited by a 780 nm laser beam, show 23-50-fold enhancement in one- and two-photon excited fluorescence in response to Ca2+, emit fourfold stronger two-photon excited fluorescence than Oregon Green 488 BAPTA-1 upon complexation with Ca2+, and can selectively detect intracellular free Ca2+ ions in live cells and living tissues with minimum interference from other metal ions and membrane-bound probes. Moreover, these probes are capable of monitoring calcium waves at a depth of 120-170 μm in live tissues for 1100-4000 s using two-photon microscopy with no artifacts of photobleaching.
Original language | English |
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Pages (from-to) | 2075-2083 |
Number of pages | 9 |
Journal | Chemistry - A European Journal |
Volume | 14 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2008 Feb 27 |
Keywords
- Calcium
- Fluorescent probes
- Imaging agents
- Live tissue
- Two-photon microscopy
ASJC Scopus subject areas
- Catalysis
- Organic Chemistry