Two-photon fluorescent probes for long-term imaging of calcium waves in live tissue

Hwan Myung Kim, Bo Ra Kim, Myoung Jin An, Jin Hee Hong, Kyoung J. Lee, Bong Rae Cho

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)


2-Acetyl-6-(dimethylamino)-naphthalene-derived two-photon fluorescent Ca2+ probes (ACa1-ACa3) are reported. They can be excited by a 780 nm laser beam, show 23-50-fold enhancement in one- and two-photon excited fluorescence in response to Ca2+, emit fourfold stronger two-photon excited fluorescence than Oregon Green 488 BAPTA-1 upon complexation with Ca2+, and can selectively detect intracellular free Ca2+ ions in live cells and living tissues with minimum interference from other metal ions and membrane-bound probes. Moreover, these probes are capable of monitoring calcium waves at a depth of 120-170 μm in live tissues for 1100-4000 s using two-photon microscopy with no artifacts of photobleaching.

Original languageEnglish
Pages (from-to)2075-2083
Number of pages9
JournalChemistry - A European Journal
Issue number7
Publication statusPublished - 2008 Feb 27


  • Calcium
  • Fluorescent probes
  • Imaging agents
  • Live tissue
  • Two-photon microscopy

ASJC Scopus subject areas

  • General Chemistry
  • Catalysis
  • Organic Chemistry


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