TY - JOUR
T1 - Two-Photon Probes for Golgi Apparatus
T2 - Detection of Golgi Apparatus in Live Tissue by Two-Photon Microscopy
AU - Choi, Ji Woo
AU - Hong, Seung Taek
AU - Kim, Mun Seok
AU - Paik, Kyu Cheol
AU - Han, Man So
AU - Cho, Bong-Rae
PY - 2019/1/1
Y1 - 2019/1/1
N2 -
We have developed blue- and yellow-emitting two-photon probes (BGolgi-blue and PGolgi-yellow) from 6-(benzo[d]oxazol-2-yl)-2-naphthalylamine and 2,5-bis(benzo[d]oxazol-2-yl)pyrazine derivatives as the fluorophores and trans-Golgi-network peptide (SDYQRL) as the Golgi-apparatus-targeting moiety. HeLa cells labeled with BGolgi-blue and PGolgi-yellow emitted two-photon-excited fluorescence at 462 and 560 nm, respectively, with effective two-photon-action cross-section values of 1860 and 1600 × 10
-50
cm
4
·s/photon, respectively. The probes can detect the Golgi apparatus in live cells and deep inside live tissue via two-photon microscopy at widely separated wavelength regions with high selectivity and minimal pH interference, and they are photostable and have low cytotoxicity.
AB -
We have developed blue- and yellow-emitting two-photon probes (BGolgi-blue and PGolgi-yellow) from 6-(benzo[d]oxazol-2-yl)-2-naphthalylamine and 2,5-bis(benzo[d]oxazol-2-yl)pyrazine derivatives as the fluorophores and trans-Golgi-network peptide (SDYQRL) as the Golgi-apparatus-targeting moiety. HeLa cells labeled with BGolgi-blue and PGolgi-yellow emitted two-photon-excited fluorescence at 462 and 560 nm, respectively, with effective two-photon-action cross-section values of 1860 and 1600 × 10
-50
cm
4
·s/photon, respectively. The probes can detect the Golgi apparatus in live cells and deep inside live tissue via two-photon microscopy at widely separated wavelength regions with high selectivity and minimal pH interference, and they are photostable and have low cytotoxicity.
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U2 - 10.1021/acs.analchem.9b00607
DO - 10.1021/acs.analchem.9b00607
M3 - Article
C2 - 30919620
AN - SCOPUS:85064344839
SN - 0003-2700
JO - Industrial And Engineering Chemistry Analytical Edition
JF - Industrial And Engineering Chemistry Analytical Edition
ER -