Two-Photon Probes for Golgi Apparatus: Detection of Golgi Apparatus in Live Tissue by Two-Photon Microscopy

Ji Woo Choi; Seung Taek Hong; Mun Seok Kim; Kyu Cheol Paik; Man So Han; Bong-Rae Cho

doi:10.1021/acs.analchem.9b00607

Two-Photon Probes for Golgi Apparatus: Detection of Golgi Apparatus in Live Tissue by Two-Photon Microscopy

Ji Woo Choi
, Seung Taek Hong
, Mun Seok Kim
, Kyu Cheol Paik
, Man So Han
, Bong-Rae Cho^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

We have developed blue- and yellow-emitting two-photon probes (BGolgi-blue and PGolgi-yellow) from 6-(benzo[d]oxazol-2-yl)-2-naphthalylamine and 2,5-bis(benzo[d]oxazol-2-yl)pyrazine derivatives as the fluorophores and trans-Golgi-network peptide (SDYQRL) as the Golgi-apparatus-targeting moiety. HeLa cells labeled with BGolgi-blue and PGolgi-yellow emitted two-photon-excited fluorescence at 462 and 560 nm, respectively, with effective two-photon-action cross-section values of 1860 and 1600 × 10 ^-50 cm ⁴ ·s/photon, respectively. The probes can detect the Golgi apparatus in live cells and deep inside live tissue via two-photon microscopy at widely separated wavelength regions with high selectivity and minimal pH interference, and they are photostable and have low cytotoxicity.

Original language	English
Journal	Analytical Chemistry
DOIs	https://doi.org/10.1021/acs.analchem.9b00607
Publication status	Published - 2019 Jan 1

ASJC Scopus subject areas

Analytical Chemistry

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title = "Two-Photon Probes for Golgi Apparatus: Detection of Golgi Apparatus in Live Tissue by Two-Photon Microscopy",

abstract = " We have developed blue- and yellow-emitting two-photon probes (BGolgi-blue and PGolgi-yellow) from 6-(benzo[d]oxazol-2-yl)-2-naphthalylamine and 2,5-bis(benzo[d]oxazol-2-yl)pyrazine derivatives as the fluorophores and trans-Golgi-network peptide (SDYQRL) as the Golgi-apparatus-targeting moiety. HeLa cells labeled with BGolgi-blue and PGolgi-yellow emitted two-photon-excited fluorescence at 462 and 560 nm, respectively, with effective two-photon-action cross-section values of 1860 and 1600 × 10 -50 cm 4 ·s/photon, respectively. The probes can detect the Golgi apparatus in live cells and deep inside live tissue via two-photon microscopy at widely separated wavelength regions with high selectivity and minimal pH interference, and they are photostable and have low cytotoxicity. ",

author = "Choi, \{Ji Woo\} and Hong, \{Seung Taek\} and Kim, \{Mun Seok\} and Paik, \{Kyu Cheol\} and Han, \{Man So\} and Bong-Rae Cho",

year = "2019",

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doi = "10.1021/acs.analchem.9b00607",

language = "English",

journal = "Analytical Chemistry",

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publisher = "American Chemical Society",

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T1 - Two-Photon Probes for Golgi Apparatus

T2 - Detection of Golgi Apparatus in Live Tissue by Two-Photon Microscopy

AU - Choi, Ji Woo

AU - Hong, Seung Taek

AU - Kim, Mun Seok

AU - Paik, Kyu Cheol

AU - Han, Man So

AU - Cho, Bong-Rae

PY - 2019/1/1

Y1 - 2019/1/1

N2 - We have developed blue- and yellow-emitting two-photon probes (BGolgi-blue and PGolgi-yellow) from 6-(benzo[d]oxazol-2-yl)-2-naphthalylamine and 2,5-bis(benzo[d]oxazol-2-yl)pyrazine derivatives as the fluorophores and trans-Golgi-network peptide (SDYQRL) as the Golgi-apparatus-targeting moiety. HeLa cells labeled with BGolgi-blue and PGolgi-yellow emitted two-photon-excited fluorescence at 462 and 560 nm, respectively, with effective two-photon-action cross-section values of 1860 and 1600 × 10 -50 cm 4 ·s/photon, respectively. The probes can detect the Golgi apparatus in live cells and deep inside live tissue via two-photon microscopy at widely separated wavelength regions with high selectivity and minimal pH interference, and they are photostable and have low cytotoxicity.

AB - We have developed blue- and yellow-emitting two-photon probes (BGolgi-blue and PGolgi-yellow) from 6-(benzo[d]oxazol-2-yl)-2-naphthalylamine and 2,5-bis(benzo[d]oxazol-2-yl)pyrazine derivatives as the fluorophores and trans-Golgi-network peptide (SDYQRL) as the Golgi-apparatus-targeting moiety. HeLa cells labeled with BGolgi-blue and PGolgi-yellow emitted two-photon-excited fluorescence at 462 and 560 nm, respectively, with effective two-photon-action cross-section values of 1860 and 1600 × 10 -50 cm 4 ·s/photon, respectively. The probes can detect the Golgi apparatus in live cells and deep inside live tissue via two-photon microscopy at widely separated wavelength regions with high selectivity and minimal pH interference, and they are photostable and have low cytotoxicity.

UR - https://www.scopus.com/pages/publications/85064344839

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U2 - 10.1021/acs.analchem.9b00607

DO - 10.1021/acs.analchem.9b00607

M3 - Article

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AN - SCOPUS:85064344839

SN - 0003-2700

JO - Analytical Chemistry

JF - Analytical Chemistry

ER -

Two-Photon Probes for Golgi Apparatus: Detection of Golgi Apparatus in Live Tissue by Two-Photon Microscopy

Abstract

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