Two-Photon Probes for Lysosomes and Mitochondria: Simultaneous Detection of Lysosomes and Mitochondria in Live Tissues by Dual-Color Two-Photon Microscopy Imaging

Chang Su Lim; Seung Taek Hong; Seong Shick Ryu; Dong Eun Kang; Bong Rae Cho

doi:10.1002/asia.201500314

Two-Photon Probes for Lysosomes and Mitochondria: Simultaneous Detection of Lysosomes and Mitochondria in Live Tissues by Dual-Color Two-Photon Microscopy Imaging

Chang Su Lim, Seung Taek Hong, Seong Shick Ryu, Dong Eun Kang, Bong Rae Cho

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Novel two-photon (TP) probes were developed for lysosomes (PLT-yellow) and mitochondria (BMT-blue and PMT-yellow). These probes emitted strong TP-excited fluorescence in cells at widely separated wavelength regions and displayed high organelle selectivity, good cell permeability, low cytotoxicity, and pH insensitivity. The BMT-blue and PLT-yellow probes could be utilized to detect lysosomes and mitochondria simultaneously in live tissues by using dual-color two-photon microscopy, with minimum interference from each other.

Original language	English
Pages (from-to)	2240-2249
Number of pages	10
Journal	Chemistry - An Asian Journal
Volume	10
Issue number	10
DOIs	https://doi.org/10.1002/asia.201500314
Publication status	Published - 2015 Oct 1

Bibliographical note

Publisher Copyright:
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords

fluorescent probes
imaging agents
lysosomes
mitochondria
two photon

ASJC Scopus subject areas

Biochemistry
Organic Chemistry

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10.1002/asia.201500314

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title = "Two-Photon Probes for Lysosomes and Mitochondria: Simultaneous Detection of Lysosomes and Mitochondria in Live Tissues by Dual-Color Two-Photon Microscopy Imaging",

abstract = "Novel two-photon (TP) probes were developed for lysosomes (PLT-yellow) and mitochondria (BMT-blue and PMT-yellow). These probes emitted strong TP-excited fluorescence in cells at widely separated wavelength regions and displayed high organelle selectivity, good cell permeability, low cytotoxicity, and pH insensitivity. The BMT-blue and PLT-yellow probes could be utilized to detect lysosomes and mitochondria simultaneously in live tissues by using dual-color two-photon microscopy, with minimum interference from each other.",

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doi = "10.1002/asia.201500314",

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AU - Hong, Seung Taek

AU - Ryu, Seong Shick

AU - Kang, Dong Eun

AU - Cho, Bong Rae

PY - 2015/10/1

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KW - imaging agents

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KW - mitochondria

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Two-Photon Probes for Lysosomes and Mitochondria: Simultaneous Detection of Lysosomes and Mitochondria in Live Tissues by Dual-Color Two-Photon Microscopy Imaging

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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