Two-Photon Probes for pH: Detection of Human Colon Cancer using Two-Photon Microscopy

Seung Taek Hong, Tae Hyeong Kim, Ji Woo Choi, Seong Jun Park, Sung An Kwon, Kyu Cheol Paik, Man So Han, Eun Sun Kim, Hoon Jai Chun, Jung Nyoung Heo, Bong Rae Cho

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11 Citations (Scopus)


We have developed two-photon (TP) pH-sensitive probes (BH-2 and BHEt-1) that exhibit absorption and emission maxima at 370 and 466 nm, and TP absorption cross-section values of 51 and 61 GM (1 GM = 10-50cm4s/photon), respectively, at 750 nm and pH 3.0 in a universal buffer (0.1 M citric acid, 0.1 M KH2PO4, 0.1 M Na2B4O7, 0.1 M Tris, 0.1 M KCl)/1,4-dioxane (7/3) solution. The TPM images of CCD-18co (a normal colon cell line) and HCT116 cells (a colon cancer cell line) labeled with BH-2 were too dim to be distinguished. When the same cells were labeled with BHEt-1, however, the TPM image of the HCT116 cells was much brighter than that of CCD-18co cells, and the relative proportion of the acidic vesicles (Pacid) of the former was 5-fold larger than that of latter. BHEt-1 could also differentiate HepG2 cells (a human liver cancer cell line) from LX-2 cells (a human hepatic stellate cell line) with a 6-fold larger Pacid value. Human colon cancer tissues labeled with BHEt-1 showed similar results, demonstrating much brighter TPM images and 6-fold larger Pacid values compared to normal tissue. These results suggest the potential utility of BHEt-1 for detecting colon cancer in human tissues using TPM.

Original languageEnglish
Pages (from-to)9830-9835
Number of pages6
JournalAnalytical chemistry
Issue number18
Publication statusPublished - 2017 Sept 19

Bibliographical note

Funding Information:
This research was supported by the National Research Foundation of the Korea (NRF) grant funded by the Korean government (NRF-2015R1A2A2A01004052) and SFC. T.H.K. and J.N.H. thank to the National Research Council of Science & Technology (NRCST) for the financial support (DRC-15-01-KRICT).

ASJC Scopus subject areas

  • Analytical Chemistry


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