Two-Photon Tracer for Human Epidermal Growth Factor Receptor-2: Detection of Breast Cancer in a Live Tissue

Ji Woo Choi; Seung Taek Hong; Dong Eun Kang; Kyu Cheol Paik; Man So Han; Chang Su Lim; Bong-Rae Cho

doi:10.1021/acs.analchem.6b00912

Two-Photon Tracer for Human Epidermal Growth Factor Receptor-2: Detection of Breast Cancer in a Live Tissue

Ji Woo Choi, Seung Taek Hong, Dong Eun Kang, Kyu Cheol Paik, Man So Han, Chang Su Lim, Bong-Rae Cho

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

We have developed a two-photon fluorescent tracer (Pyr-affibody) that shows high selectivity for human epidermal growth factor receptor-2 (HER-2). Pyr-affibody showed absorption and emission maxima at 439 and 574 nm, respectively, with a two-photon absorption cross-section value of 40 × 10^-50 cm⁴s/photon (GM) at 750 nm in aqueous buffer solution. The effective two-photon action cross-section value measured in HeLa cells was 600 GM at 730 nm, a value sufficient to obtain bright two-photon microscopy (TPM) images. Using Pyr-affibody, it was possible to detect HER-2 overexpressing cells and breast cancers at a depth of 90-130 μm in live mouse tissue by TPM.

Original language	English
Pages (from-to)	9412-9418
Number of pages	7
Journal	Analytical Chemistry
Volume	88
Issue number	19
DOIs	https://doi.org/10.1021/acs.analchem.6b00912
Publication status	Published - 2016 Oct 4

ASJC Scopus subject areas

Analytical Chemistry

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/acs.analchem.6b00912

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title = "Two-Photon Tracer for Human Epidermal Growth Factor Receptor-2: Detection of Breast Cancer in a Live Tissue",

abstract = "We have developed a two-photon fluorescent tracer (Pyr-affibody) that shows high selectivity for human epidermal growth factor receptor-2 (HER-2). Pyr-affibody showed absorption and emission maxima at 439 and 574 nm, respectively, with a two-photon absorption cross-section value of 40 × 10-50 cm4s/photon (GM) at 750 nm in aqueous buffer solution. The effective two-photon action cross-section value measured in HeLa cells was 600 GM at 730 nm, a value sufficient to obtain bright two-photon microscopy (TPM) images. Using Pyr-affibody, it was possible to detect HER-2 overexpressing cells and breast cancers at a depth of 90-130 μm in live mouse tissue by TPM.",

author = "Choi, {Ji Woo} and Hong, {Seung Taek} and Kang, {Dong Eun} and Paik, {Kyu Cheol} and Han, {Man So} and Lim, {Chang Su} and Bong-Rae Cho",

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T1 - Two-Photon Tracer for Human Epidermal Growth Factor Receptor-2

T2 - Detection of Breast Cancer in a Live Tissue

AU - Choi, Ji Woo

AU - Hong, Seung Taek

AU - Kang, Dong Eun

AU - Paik, Kyu Cheol

AU - Han, Man So

AU - Lim, Chang Su

AU - Cho, Bong-Rae

PY - 2016/10/4

Y1 - 2016/10/4

N2 - We have developed a two-photon fluorescent tracer (Pyr-affibody) that shows high selectivity for human epidermal growth factor receptor-2 (HER-2). Pyr-affibody showed absorption and emission maxima at 439 and 574 nm, respectively, with a two-photon absorption cross-section value of 40 × 10-50 cm4s/photon (GM) at 750 nm in aqueous buffer solution. The effective two-photon action cross-section value measured in HeLa cells was 600 GM at 730 nm, a value sufficient to obtain bright two-photon microscopy (TPM) images. Using Pyr-affibody, it was possible to detect HER-2 overexpressing cells and breast cancers at a depth of 90-130 μm in live mouse tissue by TPM.

AB - We have developed a two-photon fluorescent tracer (Pyr-affibody) that shows high selectivity for human epidermal growth factor receptor-2 (HER-2). Pyr-affibody showed absorption and emission maxima at 439 and 574 nm, respectively, with a two-photon absorption cross-section value of 40 × 10-50 cm4s/photon (GM) at 750 nm in aqueous buffer solution. The effective two-photon action cross-section value measured in HeLa cells was 600 GM at 730 nm, a value sufficient to obtain bright two-photon microscopy (TPM) images. Using Pyr-affibody, it was possible to detect HER-2 overexpressing cells and breast cancers at a depth of 90-130 μm in live mouse tissue by TPM.

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Two-Photon Tracer for Human Epidermal Growth Factor Receptor-2: Detection of Breast Cancer in a Live Tissue

Abstract

ASJC Scopus subject areas

UN SDGs

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